Document Detail


Effect of fish oil on levels of R- and S-enantiomers of 5-, 12-, and 15-hydroxyeicosatetraenoic acids in mouse colonic mucosa.
MedLine Citation:
PMID:  22149144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The balance of putative pro- and antiinflammatory lipoxygenase (LOX)-derived S-hydroxyeicosatetraenoic acids (S-HETEs) in colon mucosa is a potential target for modulating colon cancer risk and progression. The biological effects of S-HETEs and R-hydroxyeicosatetraenoic acids (produced by distinct pathways) may differ, but levels of these compounds in the colon are unknown. The objective of this study was to develop chiral methods to characterize hydroxyeicosatetraenoic (HETE) enantiomers in colonic mucosa and evaluate the effects of fish oil on HETE formation. C57BL/6 mice (COX-1 null, COX-2 null, wild-type) were fed a diet supplemented with either olive oil or menhaden oil for 11 wk, and R-/S-HETEs in colonic mucosa were quantified by chiral LC-MS/MS. The R-enantiomer comprised 60-72% of 5-HETE, 18-58% of 15-HETE, and 1-16% of 12-HETE in colonic mucosa, suggesting that non-LOX sources contribute to HETE profiles. Fish oil reduced levels of both R- and S-HETEs, and increased the preponderance of the R-enantiomers (particularly 12- and 15-HETEs). There was apparent shunting of arachidonic acid to 12-/15-LOX in the COX-1 null animals. This is the first report of the enantiomeric composition of HETEs in the colon in vivo and shows large effects of fish oil in the normal colon.
Authors:
Andrew P Neilson; Jianwei Ren; Yu H Hong; Ananda Sen; William L Smith; Dean E Brenner; Zora Djuric
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-12-09
Journal Detail:
Title:  Nutrition and cancer     Volume:  64     ISSN:  1532-7914     ISO Abbreviation:  Nutr Cancer     Publication Date:  2012  
Date Detail:
Created Date:  2012-01-30     Completed Date:  2012-07-06     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  7905040     Medline TA:  Nutr Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  163-72     Citation Subset:  IM    
Affiliation:
Department of Family Medicine, University of Michigan Medical School, University of Michigan, Ann Arbor, Michigan 48197, USA.
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MeSH Terms
Descriptor/Qualifier:
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / analysis,  chemistry,  metabolism*
Animals
Chromatography, High Pressure Liquid / methods*
Colon / drug effects*,  metabolism
Cyclooxygenase 1 / genetics,  metabolism
Cyclooxygenase 2 / genetics,  metabolism
Fatty Acids / analysis,  metabolism
Female
Fish Oils / pharmacology*
Hydroxyeicosatetraenoic Acids / analysis,  chemistry,  metabolism*
Intestinal Mucosa / drug effects*,  metabolism
Membrane Proteins / genetics,  metabolism
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Stereoisomerism
Grant Support
ID/Acronym/Agency:
5T32CA009676-18/CA/NCI NIH HHS; GM48864/GM/NIGMS NIH HHS; P30 CA046592/CA/NCI NIH HHS; P30-CA46592/CA/NCI NIH HHS; P50 CA130810/CA/NCI NIH HHS; R01 CA120381/CA/NCI NIH HHS; R01 CA120381/CA/NCI NIH HHS; R01 GM68848/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fish Oils; 0/Hydroxyeicosatetraenoic Acids; 0/Membrane Proteins; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 72255-35-7/5-hydroxy-6,8,11,14-eicosatetraenoic acid; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid; EC 1.14.99.-/Ptgs2 protein, mouse; EC 1.14.99.1/Cyclooxygenase 1; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/Ptgs1 protein, mouse
Comments/Corrections

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