Document Detail


Effect of Fe(3)O(4)-magnetic nanoparticles on acute exercise enhanced KCNQ(1) expression in mouse cardiac muscle.
MedLine Citation:
PMID:  20309397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
While the potential impact of magnetic nanoparticles (MNPs) has been widely explored in almost all medical fields, including cardiology, one question remains; that is whether MNPs interfere with cardiac physiological processes such as the expression and function of ion channels, especially in vivo. KCNQ(1) channels are richly expressed in cardiac myocytes and are critical to the repolarization of cardiac myocytes. In this study, we evaluated the effects of Fe(3)O(4)-magnetic nanoparticles (MNPs-Fe(3)O(4)) on the expression of KCNQ(1) in cardiac muscle of mice at rest and at different times following a single bout of swimming (SBS). Firstly, we demonstrated that the expression levels of KCNQ(1) channels are significantly up-regulated in mice following a SBS by means of reverse transcription polymerase chain reaction (RT-PCR) and western-blot. After treating mice with normal saline or pure MNPs-Fe(3)O(4) separately, we studied the potential effect of MNPs-Fe(3)O(4) on the expression profile of KCNQ(1) in mouse cardiac muscle following a SBS. A SBS increased the transcription of KCNQ(1) at 3 hours post exercise (3PE) 164% +/- 24% and at 12 hours post exercise (12PE) by 159% +/- 23% (P < 0.05), and up-regulated KCNQ(1) protein 161% +/- 27% at 12PE (P < 0.05) in saline mice. In MNPs-Fe(3)O(4) mice, KCNQ(1) mRNA increased by 151% +/- 14% and 147% +/- 12% at 3 and 12 PE, respectively (P <0.05). Meanwhile, an increase of 152% +/- 14% in KCNQ(1) protein was also detected at by 12PE. These results indicated that the administration of MNPs-Fe(3)O(4) did not cause any apparent effects on the expression profile of KCNQ(1) in rested or exercised mice cardiac muscle. Our studies suggest a novel path of KCNQ(1) current adaptations in the heart during physical exercise and in addition provide some useful information for the biomedical application of MNPs which are imperative to advance nanomedicine.
Authors:
Lijie Liu; Baoan Chen; Feixiang Teng; Lijuan Shi; Nan Jing; Li Wang; Ningna Chen; Guohua Xia; Xiaomao Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-09
Journal Detail:
Title:  International journal of nanomedicine     Volume:  5     ISSN:  1178-2013     ISO Abbreviation:  Int J Nanomedicine     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-23     Completed Date:  2010-05-17     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101263847     Medline TA:  Int J Nanomedicine     Country:  New Zealand    
Other Details:
Languages:  eng     Pagination:  109-16     Citation Subset:  IM    
Affiliation:
Department of Physiology and Pharmacology, Medical School, Southeast University, Nanjing 210009, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Electromagnetic Fields
Ferric Compounds / administration & dosage*,  chemistry
Gene Expression / drug effects,  physiology
Heart / drug effects,  physiology*
KCNQ1 Potassium Channel / metabolism*
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Nanoparticles / administration & dosage*,  chemistry,  ultrastructure
Particle Size
Physical Exertion / drug effects,  physiology*
Chemical
Reg. No./Substance:
0/Ferric Compounds; 0/KCNQ1 Potassium Channel; 0/Kcnq1 protein, mouse; 1309-37-1/ferric oxide
Comments/Corrections

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