Document Detail

Effect of Exercise on the Skeletal Muscle Proteome in Patients with Type 2 Diabetes.
MedLine Citation:
PMID:  23274603     Owner:  NLM     Status:  Publisher    
PURPOSE: Exercise training alters protein abundance in muscle of healthy individuals, but the effect of exercise on these proteins in patients with type 2 diabetes (T2D) is unknown. The aim of this study was to determine how exercise training alters the skeletal muscle proteome in patients with T2D. METHODS: Biopsies of the vastus lateralis were obtained before and after 4 weeks of exercise training in six patients with T2D (54 ± 4 yrs, BMI 29± 2) and six age- and BMI- matched control subjects (48 ± 2, BMI 28 ± 3) studied at baseline. Proteins were identified and quantified using normalized spectral abundance factors (NSAF) by multidimensional high-resolution mass spectrometry. RESULTS: Of 1,329 proteins assigned at baseline, 438 were present in at least half of all muscle samples; of these, 15 proteins differed significantly between patients with T2D and control subjects (p<0.05). In the diabetic patients, exercise training altered the abundance of 17 proteins (p<0.05). Key training adaptations included an increase in proteins of the malate-aspartate shuttle and citric acid cycle, reduced abundance of glycolytic proteins, and altered abundance of cytoskeleton proteins. CONCLUSIONS: The data from this study support the ability of exercise training to alter the abundance of proteins that regulate metabolism and cytoskeletal structure in patients with T2D. These findings open new avenues for future research.
Sophie E Hussey; Carrie G Sharoff; Andrew Garnham; Yi Zhengping; Benjamin P Bowen; Lawrence J Mandarino; Mark Hargreaves
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-27
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  -     ISSN:  1530-0315     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1Department of Physiology, The University of Melbourne, VIC, Australia 2School of Exercise and Nutrition Sciences, Deakin University, VIC, Australia 3Center for Metabolic and Vascular Biology, Arizona State University, Tempe, AZ 4Mayo Clinic, Scottsdale, AZ 5School of Life Sciences, Arizona State University, Tempe, AZ.
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