Document Detail


Effect of exercise on the skeletal muscle proteome in patients with type 2 diabetes.
MedLine Citation:
PMID:  23274603     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Exercise training alters protein abundance in the muscle of healthy individuals, but the effect of exercise on these proteins in patients with type 2 diabetes (T2D) is unknown. The aim of this study was to determine how exercise training alters the skeletal muscle proteome in patients with T2D.
METHODS: Biopsies of the vastus lateralis were obtained before and after 4 wk of exercise training in six patients with T2D (54 ± 4 yr; body mass index (BMI), 29 ± 2) and six age- and BMI-matched control subjects (48 ± 2; BMI, 28 ± 3) studied at the baseline. The proteins were identified and quantified using normalized spectral abundance factors by multidimensional high-resolution mass spectrometry.
RESULTS: Of the 1329 proteins assigned at the baseline, 438 were present in at least half of all the muscle samples; of these, 15 proteins differed significantly between the patients with T2D and control subjects (P < 0.05). In the diabetic patients, the exercise training altered the abundance of 17 proteins (P < 0.05). Key training adaptations included an increase in proteins of the malate-aspartate shuttle and citric acid cycle, reduced the abundance of glycolytic proteins, and altered the abundance of cytoskeleton proteins.
CONCLUSION: The data from this study support the ability of exercise training to alter the abundance of proteins that regulate metabolism and cytoskeletal structure in patients with T2D. These findings open new avenues for future research.
Authors:
Sophie E Hussey; Carrie G Sharoff; Andrew Garnham; Zhengping Yi; Benjamin P Bowen; Lawrence J Mandarino; Mark Hargreaves
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  45     ISSN:  1530-0315     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-20     Completed Date:  2014-02-03     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1069-76     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Case-Control Studies
Chromatography, High Pressure Liquid
Diabetes Mellitus, Type 2 / metabolism*
Exercise / physiology*
Exercise Test
Female
Humans
Male
Middle Aged
Muscle Proteins / metabolism*
Proteome / metabolism*
Quadriceps Muscle / metabolism*
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Grant Support
ID/Acronym/Agency:
R01 DK047936/DK/NIDDK NIH HHS; R01 DK066483/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Muscle Proteins; 0/Proteome
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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