Document Detail


Effect of Erythrogen on post-thaw recovery of cryopreserved cell suspensions of indica rice (Oryza sativa L.).
MedLine Citation:
PMID:  11788878     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study shows that adding haemoglobin solution (Erythrogen) to post-thaw medium of Indica rice (Oryza sativa L.) cells enhances survival following cryopreservation. Haemoglobin (1:50 - 1:200 v:v) had a beneficial effect on post-thaw viability and subsequent cell growth. A key finding was that the successful recovery from cryopreservation of cell suspensions of the Indica rice cvs. BR26 and Pajam, and their re-establishment in AA2 medium, reflected a requirement for such supplementation of the post-thaw recovery medium with Erythrogen.
Authors:
M Al-Forkan; P Anthony; J B Power; M R Davey; K C Lowe
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  22     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2001 Nov-Dec
Date Detail:
Created Date:  2002-01-14     Completed Date:  2002-03-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  367-74     Citation Subset:  IM    
Affiliation:
Plant Sciences Division, School of Biosciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
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MeSH Terms
Descriptor/Qualifier:
Cell Survival / drug effects
Cells, Cultured
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Culture Media, Conditioned / pharmacology
Hemoglobins / pharmacology*
Oryza sativa / cytology*,  growth & development
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Culture Media, Conditioned; 0/Hemoglobins; 0/erythrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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