Document Detail

Effect of docosahexaenoic acid on quality of cryopreserved boar semen in different breeds.
MedLine Citation:
PMID:  19090818     Owner:  NLM     Status:  MEDLINE    
During the cryopreservation process, the level of polyunsaturated fatty acids, especially docosahexaenoic acid (DHA), in the sperm plasma membrane decreases significantly because of lipid peroxidation, which may contribute to sperm loss quality (i.e. fertility) of frozen-thawed semen. The aim of this study was to investigate the effect of supplementation of DHA (fish oil) in freezing extender II on frozen-thawed semen quality. Semen from 20 boars of proven motility and morphology, were used in this study. Boar semen was split into four groups, in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various levels of fish oil to reach DHA level of 1X (group I, control, no added fish oil), 6X (group II), 12X (group III) and 18X (group IV). Semen solutions were frozen by using a controlled rate freezer. After cryopreservation, frozen semen was thawed and evaluated for progressive motility, viability by using SYBR-14/Ethidiumhomodimer-1 (EthD-1) staining and acrosome integrity by using FITC-PNA/EthD-1 staining. There was a significantly higher (p < 0.001) percentage of progressive motility, viability and acrosome integrity in DHA (fish oil) supplemented groups than control group. Generally, there seemed to be a dose-dependent effect of DHA, with the highest percentage of progressive motility, viability and acrosome integrity in group-III. In conclusion, supplementation of the LEY extender with DHA by adding fish oil was effective for freezing boar semen as it resulted in higher post-thaw plasma membrane integrity and progressive motility.
K Kaeoket; P Sang-urai; A Thamniyom; P Chanapiwat; M Techakumphu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-15
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  45     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-30     Completed Date:  2010-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  458-63     Citation Subset:  IM    
Faculty of Veterinary Science, Mahidol University, Nakorn-pathom, Thailand.
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MeSH Terms
Acrosome / physiology
Cell Membrane / physiology
Cell Survival
Cryopreservation / methods,  veterinary*
Docosahexaenoic Acids / pharmacology*
Fish Oils / administration & dosage
Semen / physiology*
Semen Preservation / veterinary*
Species Specificity
Sperm Motility
Spermatozoa / abnormalities,  physiology*,  ultrastructure
Swine / physiology*
Reg. No./Substance:
0/Fish Oils; 25167-62-8/Docosahexaenoic Acids

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