| Effect of Disulfide Interactions and Hydrolysis on the Thermal Aggregation of β-Lactoglobulin. | |
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MedLine Citation:
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PMID: 20812724 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The roles of sulfhydryl/disulfide interactions and acid/pepsin hydrolysis on β-lactoglobulin (β-lg) thermal aggregation at acidic pH 3.35 and 2 were studied using rheology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transmission electron microscopy (TEM), and Western blotting. Pepsin promoted additional hydrolysis compared to the acid-hydrolyzed control sample based on a 12% increase in free amino groups. Hydrolysis with pepsin also resulted in an increase in the apparent viscosity by 2 logs upon heating 8% β-lg solutions at pH 3.35. Seemingly, hydrolysis promoted thermal aggregation of β-lg, correlating well with viscosity increases. Large microgels were observed in heated pepsin hydrolysates using TEM, supporting the increased viscosities of these dispersions. During thermal aggregation (85 °C, 3 h) of β-lg at pH 3.35, beyond the existence of limited disulfide interactions, acid hydrolysis and noncovalent interactions more likely play a crucial role in defining the functionality of acidified powdered modified whey ingredients. |
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Authors:
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Prashant Mudgal; Christopher R Daubert; Debra A Clare; E Allen Foegeding |
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Publication Detail:
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Type: Journal Article Date: 2010-09-02 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 59 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1491-7 Citation Subset: IM |
Affiliation:
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Department of Food, Bioprocessing and Nutrition Sciences, Schaub Hall, Dan Allen Drive, North Carolina State University , Raleigh, North Carolina 27695-7624, United States. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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