| Effect of different classes of gadolinium-based contrast agents on control and nephrogenic systemic fibrosis-derived fibroblast proliferation. | |
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MedLine Citation:
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PMID: 20663970 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To determine the ability of different types of gadolinium-based contrast agents (GBCAs) to stimulate fibroblast proliferation in monolayer cell culture. MATERIALS AND METHODS: The National Health Service West Glasgow Ethics Committee granted approval for this study. Fibroblasts established from healthy volunteers (control subjects) and from lesional skin of patients with nephrogenic systemic fibrosis were exposed to a range of concentrations of ionic and nonionic linear and macrocyclic contrast agents over 4 days, and the effect on growth was determined. The lowest concentration of contrast agent that stimulated the maximum effect on fibroblast growth was selected for determination of its effect on fibroblast growth over 8 days. The effect of contrast agents on hyaluronan and collagen synthesis was determined with an enzyme-linked immunosorbent assay. Responses were assessed with analysis of variance (general linear model). RESULTS: The linear gadolinium contrast agents (gadodiamide, gadoversetamide, gadopentetate dimeglumine, and gadobenate dimeglumine) produced a maximum stimulation of fibroblast proliferation at a concentration of 0.1 mmol/L, with cell numbers increasing up to 2.3-fold. The macrocyclic contrast agents (gadoteric acid and gadoteridol) produced a maximum stimulation of fibroblast proliferation at a concentration of 5 mmol/L. The reference gadolinium agents (N-methylglucamine gadolinium ethylenediaminetetraacetic acid and gadolinium trichloride) stimulated fibroblast proliferation at a concentration of 0.01 mmol/L and were toxic at a concentration greater than 1 mmol/L. Growth curves supported the dose-response observations. Hyaluronan synthesis was stimulated by gadoversetamide, gadobenate dimeglumine, gadodiamide, and gadopentetate dimeglumine at a concentration of 0.1 mmol/L and by gadolinium trichloride at a concentration of 0.01 mmol/L, whereas collagen synthesis was unaffected. Conclusion: This study provides evidence that different classes of gadolinium chelates stimulate human fibroblast proliferation. |
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Authors:
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Michael Edward; Jean A Quinn; A David Burden; Ben B Newton; Alan G Jardine |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-27 |
Journal Detail:
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Title: Radiology Volume: 256 ISSN: 1527-1315 ISO Abbreviation: Radiology Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-19 Completed Date: 2010-09-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0401260 Medline TA: Radiology Country: United States |
Other Details:
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Languages: eng Pagination: 735-43 Citation Subset: AIM; IM |
Copyright Information:
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(c) RSNA, 2010. |
Affiliation:
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Section of Dermatology, Division of Cancer Sciences, and Glasgow Cardiovascular Centre, Faculty of Medicine, University of Glasgow, Glasgow G12 8QQ, Scotland. m.edward@clinmed.gla.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cell Proliferation Collagen / biosynthesis Contrast Media / pharmacology* Dose-Response Relationship, Drug Edetic Acid / pharmacology Enzyme-Linked Immunosorbent Assay Fibroblasts / drug effects*, metabolism Gadolinium / pharmacology* Gadolinium DTPA / pharmacology Heterocyclic Compounds / pharmacology Humans Hyaluronic Acid / biosynthesis Linear Models Magnetic Resonance Imaging Meglumine / analogs & derivatives, pharmacology Middle Aged Nephrogenic Fibrosing Dermopathy / chemically induced Organometallic Compounds / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 0/Heterocyclic Compounds; 0/Organometallic Compounds; 0/gadoversetamide; 112188-16-6/gadoteridol; 113662-23-0/gadobenic acid; 122795-43-1/gadodiamide; 15213-88-4/gadolinium EDTA; 60-00-4/Edetic Acid; 6284-40-8/Meglumine; 7440-54-2/Gadolinium; 80529-93-7/Gadolinium DTPA; 9004-61-9/Hyaluronic Acid; 9007-34-5/Collagen; 92923-44-9/gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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