| The effect of DYS-14 copy number variations on extracellular fetal DNA quantification in maternal circulation. | |
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MedLine Citation:
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PMID: 19456250 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aims of our research involved to investigate DYS-14 copy number variations in healthy males, to quantify extracellular DNA in maternal circulation in normal versus complicated pregnancies, and to study variations in the DYS-14 copy number in extracellular male fetal DNA. Fifty-five healthy males, 43 uncomplicated male singleton pregnancies (23 sampled at the 16th week and 20 sampled at the 36th week), and 15 pregnancies with placental insufficiency (PI)-related complications (mean 34.1 weeks) were analyzed using real-time PCR with DYS-14 sequence, sex determining region Y (SRY), and beta-globin (GLO) genes used as markers. Increased levels of extracellular DNA were detected in PI-related complications relative to gestational age-matched controls (SRY, p < 0.001; DYS-14, p = 0.007; GLO, p < 0.001). When the mean + 2SD (standard deviation) of controls was used as a cutoff, SRY, DYS-14, and GLO achieved 91.7%, 68.8%, and 94.4% accuracy, respectively, for differentiation between normal and complicated pregnancies. Considerable variations in the DYS-14 copy number in healthy males (mean 52.6) and extracellular DNA were found. A lower DYS-14 copy number was observed in PI-related complications (mean 83.5) compared to uncomplicated pregnancies (16th week: mean 114.2, p = 0.02; 36th week: mean 142.8, p = 0.04). The DYS-14 copy number was higher in extracellular DNA throughout gestation relative to healthy males. We concluded that, regarding interindividual copy number variations, the DYS-14 sequence is not an optimal marker for extracellular fetal DNA quantification for differentiation between normal and complicated pregnancies. |
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Authors:
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Ilona Hromadnikova; Martina Benesova; Lenka Zejskova; Jana Stehnova; Jindrich Doucha; Petr Sedlacek; Klara Dlouha; Ladislav Krofta |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: DNA and cell biology Volume: 28 ISSN: 1557-7430 ISO Abbreviation: DNA Cell Biol. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-24 Completed Date: 2009-07-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9004522 Medline TA: DNA Cell Biol Country: United States |
Other Details:
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Languages: eng Pagination: 351-8 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Cell Pathology, 3rd Medical Faculty, Charles University, Prague, Czech Republic. ilona.hromadnikova@lf3.cuni.cz |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Case-Control Studies Cell Cycle Proteins / genetics* Child Child, Preschool Chromosomes, Human, Y / genetics* DNA / blood* Female Fetus* Gene Dosage* Gestational Age Humans Infant Male Middle Aged Mothers Placental Insufficiency / diagnosis, genetics* Pregnancy Pregnancy Outcome Prenatal Diagnosis* Sex-Determining Region Y Protein / genetics Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/SRY protein, human; 0/Sex-Determining Region Y Protein; 0/TSPY1 protein, human; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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