| Effect of cytotoxic agents and retinoic acid on Myc-N protein expression in neuroblastoma. | |
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MedLine Citation:
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PMID: 19550295 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: Neuroblastoma is an important pediatric tumor in which Myc-N amplification is a well-known poor prognostic indicator. It has a great diversity in clinical behavior. The effect of pharmacologic agents used in neuroblastoma treatment on Myc-N expression is still unclear. METHOD: We analyzed Myc-N expression changes by immunocytochemistry in Myc-N-positive Kelly human neuroblastoma cell line using retinoic acid and cytotoxic drugs (cisplatin, vincristine, cyclophosphamide, etoposide, and doxorubicin) and their combinations compared with control conditions. First, concentration of drugs were determined as LD50 doses. Kelly cells and drugs were incubated for 24 hours in 5% CO2, 37 degrees C in 96-well plates. Myc-N expression was scored semiquantitatively as negative, mild, moderate, or high positive. RESULTS: Myc-N amplification did not change with any agent or combination. It was higher than 20 copies in all conditions. Myc-N protein expression was high in control and doxorubicin group. It was moderate in retinoic acid, cyclophosphamide, retinoic acid combined with cyclophosphamide and retinoic acid combined with doxorubicin groups. The expression was mild in cisplatin, vincristine, etoposide, retinoic acid combined with etoposide, and retinoic acid combined with cisplatin groups. Myc-N expression was negative in retinoic acid combined with vincristine group. CONCLUSIONS: Myc-N expression is reduced with cytotoxic agents and retinoic acid in neuroblastoma although Myc-N amplification remains the same. Retinoic acid combined with vincristine is the most effective combination to reduce Myc-N expression. Our results suggest that therapeutic applications of these agents as low dose maintenance therapy might be useful. |
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Authors:
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Safiye Aktas; Zekiye Altun; Zubeyde Erbayraktar; Nevim Aygun; Nur Olgun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry Volume: 18 ISSN: 1533-4058 ISO Abbreviation: Appl. Immunohistochem. Mol. Morphol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-23 Completed Date: 2010-04-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888796 Medline TA: Appl Immunohistochem Mol Morphol Country: United States |
Other Details:
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Languages: eng Pagination: 86-9 Citation Subset: IM |
Affiliation:
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Institute of Oncology, Dokuz Eylul University, Izmir, Turkey. safiyeaktas@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Combined Chemotherapy Protocols
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pharmacology* Cell Line, Tumor Child Gene Amplification / drug effects Gene Expression / drug effects Humans Neuroblastoma / drug therapy*, metabolism, pathology Proto-Oncogene Proteins c-myc / analysis, drug effects* Tretinoin / pharmacology* Vincristine / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/MYC protein, human; 0/Proto-Oncogene Proteins c-myc; 302-79-4/Tretinoin; 57-22-7/Vincristine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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