| Effect of Curcumin on miRNA Expression in Human Y79 Retinoblastoma Cells. | |
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MedLine Citation:
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PMID: 22510010 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Purpose: Retinoblastoma (RB) is the most common intraocular malignancy in children. Deregulation of several miRNAs has been identified in RB, suggesting a potential role in tumorigenesis. Recent evidence suggests that many dietary components like folate, retinoids and curcumin act as potential anticancer/antiproliferative agents by regulating the expression of miRNA. In this study, we investigated the effect of phenolic compound curcumin on miRNA expression in Y79 RB cells. Materials and Methods: We analyzed the expression profile of miRNA by microarray analysis and quantitative real-time polymerase chain reaction (qRT-PCR) in curcumin-treated Y79 RB cells. Transfection of miR-22 was performed using Lipofectamine 2000. Cell viability, in vitro scratch migration assay, prediction of miRNA targets and Western blot analysis were performed to determine the biological function of miR-22 in Y79 RB cells. Results: In Y79 RB cells treated with curcumin, 5 human miRNAs were upregulated and 16 were downregulated as detected with the miRNA microarray analysis. miR-22, a tumor-suppressor miRNA was one of the miRNA which was upregulated by curcumin. Transfected miR-22 Y79 cells inhibited the cell proliferation and reduced the migration, and erythoblastic leukemia viral oncogene homolog 3 (Erbb3) was confirmed to be the target gene of miR-22. Conclusion: These observations suggest that curcumin modulate the miRNA expression profile, thereby exerting its anticancer effects on RB cells. |
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Authors:
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Seethalakshmi Sreenivasan; Karthiyaini Thirumalai; Ravikanth Danda; Subramanian Krishnakumar |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current eye research Volume: 37 ISSN: 1460-2202 ISO Abbreviation: Curr. Eye Res. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-04-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 421-8 Citation Subset: IM |
Affiliation:
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L&T Department of Ocular Pathology, Vision Research Foundation , Sankara Nethralaya, 18 College Road, Nungambakkam, Chennai - 600 006. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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