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Effect of Curcumin on miRNA Expression in Human Y79 Retinoblastoma Cells.
MedLine Citation:
PMID:  22510010     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Purpose: Retinoblastoma (RB) is the most common intraocular malignancy in children. Deregulation of several miRNAs has been identified in RB, suggesting a potential role in tumorigenesis. Recent evidence suggests that many dietary components like folate, retinoids and curcumin act as potential anticancer/antiproliferative agents by regulating the expression of miRNA. In this study, we investigated the effect of phenolic compound curcumin on miRNA expression in Y79 RB cells. Materials and Methods: We analyzed the expression profile of miRNA by microarray analysis and quantitative real-time polymerase chain reaction (qRT-PCR) in curcumin-treated Y79 RB cells. Transfection of miR-22 was performed using Lipofectamine 2000. Cell viability, in vitro scratch migration assay, prediction of miRNA targets and Western blot analysis were performed to determine the biological function of miR-22 in Y79 RB cells. Results: In Y79 RB cells treated with curcumin, 5 human miRNAs were upregulated and 16 were downregulated as detected with the miRNA microarray analysis. miR-22, a tumor-suppressor miRNA was one of the miRNA which was upregulated by curcumin. Transfected miR-22 Y79 cells inhibited the cell proliferation and reduced the migration, and erythoblastic leukemia viral oncogene homolog 3 (Erbb3) was confirmed to be the target gene of miR-22. Conclusion: These observations suggest that curcumin modulate the miRNA expression profile, thereby exerting its anticancer effects on RB cells.
Authors:
Seethalakshmi Sreenivasan; Karthiyaini Thirumalai; Ravikanth Danda; Subramanian Krishnakumar
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current eye research     Volume:  37     ISSN:  1460-2202     ISO Abbreviation:  Curr. Eye Res.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8104312     Medline TA:  Curr Eye Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  421-8     Citation Subset:  IM    
Affiliation:
L&T Department of Ocular Pathology, Vision Research Foundation , Sankara Nethralaya, 18 College Road, Nungambakkam, Chennai - 600 006.
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