Document Detail


Effect of common cryoprotectants on critical warming rates and ice formation in aqueous solutions.
MedLine Citation:
PMID:  22728046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ice formation on warming is of comparable or greater importance to ice formation on cooling in determining survival of cryopreserved samples. Critical warming rates required for ice-free warming of vitrified aqueous solutions of glycerol, dimethyl sulfoxide, ethylene glycol, polyethylene glycol 200 and sucrose have been measured for warming rates of order 10-10⁴ K/s. Critical warming rates are typically one to three orders of magnitude larger than critical cooling rates. Warming rates vary strongly with cooling rates, perhaps due to the presence of small ice fractions in nominally vitrified samples. Critical warming and cooling rate data spanning orders of magnitude in rates provide rigorous tests of ice nucleation and growth models and their assumed input parameters. Current models with current best estimates for input parameters provide a reasonable account of critical warming rates for glycerol solutions at high concentrations/low rates, but overestimate both critical warming and cooling rates by orders of magnitude at lower concentrations and larger rates. In vitrification protocols, minimizing concentrations of potentially damaging cryoprotectants while minimizing ice formation will require ultrafast warming rates, as well as fast cooling rates to minimize the required warming rates.
Authors:
Jesse B Hopkins; Ryan Badeau; Matthew Warkentin; Robert E Thorne
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-06-19
Journal Detail:
Title:  Cryobiology     Volume:  65     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-16     Completed Date:  2013-02-11     Revised Date:  2013-12-05    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  169-78     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Cryopreservation
Cryoprotective Agents / chemistry*
Dimethyl Sulfoxide / chemistry
Ethylene Glycol / chemistry
Freezing
Glycerol / chemistry
Heating
Ice / analysis*
Polyethylene Glycols / chemistry
Vitrification
Water / chemistry*
Grant Support
ID/Acronym/Agency:
R01 GM065981/GM/NIGMS NIH HHS; R01 GM65981/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Ice; 0/Polyethylene Glycols; 059QF0KO0R/Water; FC72KVT52F/Ethylene Glycol; PDC6A3C0OX/Glycerol; YOW8V9698H/Dimethyl Sulfoxide
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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