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Effect of Cholesterol Deposition on Bacterial Adhesion to Contact Lenses.
MedLine Citation:
PMID:  21552177     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
PURPOSE.: To examine the effect of cholesterol on the adhesion of bacteria to silicone hydrogel contact lenses. METHODS.: Contact lenses, collected from subjects wearing Acuvue Oasys or PureVision lenses, were extracted in chloroform:methanol (1:1, v/v) and amount of cholesterol was estimated by thin-layer chromatography. Unworn lenses were soaked in cholesterol, and the numbers of Pseudomonas aeruginosa strains or Staphylococcus aureus strains that adhered to the lenses were measured. Cholesterol was tested for effects on bacterial growth by incubating bacteria in medium containing cholesterol. RESULTS.: From ex vivo PureVision lenses, 3.4 ± 0.3 μg/lens cholesterol was recovered, and from Acuvue Oasys lenses, 2.4 ± 0.2 to 1.0 ± 0.1 μg/lens cholesterol was extracted. Cholesterol did not alter the total or viable adhesion of any strain of P. aeruginosa or S. aureus (p > 0.05). However, worn PureVision lenses reduced the numbers of viable cells of P. aeruginosa (5.8 ± 0.4 log units) compared with unworn lenses (6.4 ± 0.2 log units, p = 0.001). Similarly, there were fewer numbers of S. aureus 031 adherent to worn PureVision (3.05 ± 0.8 log units) compared with unworn PureVision (4.6 ± 0.3 log units, p = 0.0001). Worn Acuvue Oasys lenses did not affect bacterial adhesion. Cholesterol showed no effect on the growth of any test strain. CONCLUSIONS.: Although cholesterol has been shown to adsorb to contact lenses during wear, this lipid does not appear to modulate bacterial adhesion to a lens surface.
Authors:
Negar Babaei Omali; Hua Zhu; Zhenjun Zhao; Jerome Ozkan; Banglao Xu; Roya Borazjani; Mark D P Willcox
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-5
Journal Detail:
Title:  Optometry and vision science : official publication of the American Academy of Optometry     Volume:  -     ISSN:  1538-9235     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8904931     Medline TA:  Optom Vis Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
*BOptom †PhD Brien Holden Vision Institute (NBO, HZ, ZZ, JO, BX, MDPW), School of Optometry and Vision Science, University of New South Wales (NBO, HZ, MDPW), Sydney, New South Wales, Australia, and Research, Alcon Labs, Fort Worth, Texas (RB).
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