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Effect of Cerebral Hypothermia and Asphyxia on the Subventricular Zone and White Matter Tracts in Preterm Fetal Sheep.
MedLine Citation:
PMID:  22765912     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Prolonged, moderate cerebral hypothermia is consistently neuroprotective after experimental hypoxia-ischemia. We have previously shown that hypothermia is also protective after profound asphyxia in the preterm brain. However, there is concern whether hypothermia could suppress the proliferative response to injury in the white matter or subventricular zone (SVZ). Preterm (0.7 gestation) fetal sheep received complete umbilical cord occlusion for 25min followed by cerebral hypothermia (extradural temperature reduced from 39.4±0.3 to 29.5±2.6°C) from 90min to 70h after the end of occlusion or sham cooling. Occlusion-normothermia was associated with no effect on CNPase+ cells, but loss of O4+ oligodendrocytes, induction of cleaved caspase-3, and IB4+ microglia in the gyral and periventricular white matter compared to sham-occlusion (p<0.05), with a significant increase in KI67+cells in the periventricular white matter (p<0.05). Hypothermia was associated with significant protection of O4+cells, with suppression of IB4+ microglia and KI67+cells in the periventricular white matter. There was no significant change in astrocytes, microglia, KI67+, or caspase-3+cells in the SVZ after asphyxia. In conclusion, this study provides strong support for the selective vulnerability of immature oligodendrocytes to a highly relevant insult in the fetal sheep. Although white matter protection with cerebral hypothermia was associated with reduced proliferation in the white matter tracts, it did not impair proliferation in the SVZ.
Authors:
Robert Daniel Barrett; Laura Bennet; Andrew Naylor; Sherly A George; Justin M Dean; Alistair Jan Gunn
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-2
Journal Detail:
Title:  Brain research     Volume:  -     ISSN:  1872-6240     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
The University of Auckland, Department of Physiology, Faculty of Medical and Health Sciences, Private Bag 92019, Auckland 1023, New Zealand.
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