Document Detail


The effect of centrifugal forces on viability of adipocytes in centrifuged lipoaspirates.
MedLine Citation:
PMID:  20798633     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Standard protocols for autologous fat transfer involve centrifugation of the lipoaspirate for better separation. The effect of the centrifugal forces on cell viability and possible induction of apoptosis is discussed controversially in current literature. We have examined the vitality of adipocytes in lipoaspirates after centrifugation by 8 different accelerations up to 20.000g immediately after the respective centrifugation as well as 4 days later by staining. No significant alterations in the viability of differently centrifuged adipocytes were found. Cultivation of the samples after centrifugation revealed no apoptotic changes. Our results show that centrifugation, as a part of the protocol of lipofilling, has no effect on the survival rate of isolated adipocytes in the purified fat. In contrast, lipoaspirates centrifuged with higher accelerations seem to be better cleansed of oil and cell debris than samples treated with lower centrifugal forces.
Authors:
Ann K Pulsfort; Timm P Wolter; Norbert Pallua
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of plastic surgery     Volume:  66     ISSN:  1536-3708     ISO Abbreviation:  Ann Plast Surg     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7805336     Medline TA:  Ann Plast Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  292-5     Citation Subset:  IM    
Affiliation:
From the Department of Plastic and Reconstructive Surgery, Hand Surgery-Burn Center, University Hospital of the RWTH, Pauwelsstrasse 30, D-52074 Aachen, Germany.
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