| The effect of centrifugal forces on viability of adipocytes in centrifuged lipoaspirates. | |
| | |
MedLine Citation:
|
PMID: 20798633 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Standard protocols for autologous fat transfer involve centrifugation of the lipoaspirate for better separation. The effect of the centrifugal forces on cell viability and possible induction of apoptosis is discussed controversially in current literature. We have examined the vitality of adipocytes in lipoaspirates after centrifugation by 8 different accelerations up to 20.000g immediately after the respective centrifugation as well as 4 days later by staining. No significant alterations in the viability of differently centrifuged adipocytes were found. Cultivation of the samples after centrifugation revealed no apoptotic changes. Our results show that centrifugation, as a part of the protocol of lipofilling, has no effect on the survival rate of isolated adipocytes in the purified fat. In contrast, lipoaspirates centrifuged with higher accelerations seem to be better cleansed of oil and cell debris than samples treated with lower centrifugal forces. |
| | |
Authors:
|
Ann K Pulsfort; Timm P Wolter; Norbert Pallua |
Related Documents
:
|
21122473 - Targeting b cells for the treatment of sle: the beginning of the end or the end of the ... 7229093 - Comparative studies of spermatogenesis in fertile and subfertile men. 17553883 - A protein kinase a-dependent mechanism by which rotavirus affects the distribution and ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Annals of plastic surgery Volume: 66 ISSN: 1536-3708 ISO Abbreviation: Ann Plast Surg Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-02-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7805336 Medline TA: Ann Plast Surg Country: United States |
Other Details:
|
Languages: eng Pagination: 292-5 Citation Subset: IM |
Affiliation:
|
From the Department of Plastic and Reconstructive Surgery, Hand Surgery-Burn Center, University Hospital of the RWTH, Pauwelsstrasse 30, D-52074 Aachen, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A treatment strategy for postburn neck reconstruction: emphasizing the functional and aesthetic impo...
Next Document: Transversus abdominis plane block reduces the analgesic requirements after abdominoplasty with flank...