Document Detail


Effect of Cbfa1 on osteogenic differentiation of mesenchymal stem cells under hypoxia condition.
MedLine Citation:
PMID:  24753746     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia.
METHODS: The second passage of the MSCs were transfected with Cbfa1 and then cultured in 20% O2 and 3% O2 condition individually. The biological features of the cultured MSCs were assessed by the Real-time PCR.
RESULTS: After transfected with Cbfa1, the morphology of MSCs was no significant difference between two oxygen concentrations; The RT-PCR examination revealed that the expression of Cbfa1, BMP, OPN and VEGF in MSCs was higher than those before Cbfa1 transfection (P<0.05), especial the expression of Cbfa1 (P<0.05).
CONCLUSION: After transfected with Cbfa1, cell morphology or growth cycle of MSCs was not significantly affected, but its osteogenic differentiation potential enhanced, particularly its osteogenic differentiation potential under hypoxia enhanced.
Authors:
Zhang-Hua Li; Wen Liao; Qiang Zhao; Tang Huan; Pan Feng; Xia Wei; Yang Yi; Ning-Sheng Shao
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Publication Detail:
Type:  Journal Article     Date:  2014-03-15
Journal Detail:
Title:  International journal of clinical and experimental medicine     Volume:  7     ISSN:  1940-5901     ISO Abbreviation:  Int J Clin Exp Med     Publication Date:  2014  
Date Detail:
Created Date:  2014-04-22     Completed Date:  2014-04-22     Revised Date:  2014-04-24    
Medline Journal Info:
Nlm Unique ID:  101471010     Medline TA:  Int J Clin Exp Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  540-8     Citation Subset:  -    
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