Document Detail


Effect of β -Catenin siRNA on Proliferation and Apoptosis of Hepatoma Cell Line SMMC-7721 and HepG-2.
MedLine Citation:
PMID:  22086699     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background/Aims: The aim of this study was to investigate the effect of expression of siRNA β- catenin silent gene on cell cycle and apoptosis of different hepatoma cells. Methodology: The effect of the β-catenin-siRNA recombinant plasmid on the growth of different hepatoma cells was observed by MTT assays; and the effect of the recombinant plasmid on proliferation and apoptosis of different hepatoma cells was detected through flow cytometry. Results: Transfection of the β-catenin-siRNA recombinant plasmid into hepatoma cells significantly inhibited β-catenin expression at protein and mRNA levels (p<0.05) as shown by western blotting and RT-PCR, respectively. The MTT assay showed that β-catenin-siRNA can significantly inhibit growth of hepatoma cells. The results of flow cytometry showed that the growth of different hepatoma cells was blocked at G0/G1 phase and compared with the control group there was a significant difference (p<0.05). Flow cytometry also showed that apoptosis of different hepatoma cells, compared with the control group, was significantly different (p<0.05). Conclusions: Inhibition of β-catenin in hepatoma cells can inhibit growth and promote apoptosis, and there was difference in the effect on proliferation and apoptosis between different hepatoma cells.
Authors:
Wang Liyan; Sun Xun; Meng Xiangwei
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Hepato-gastroenterology     Volume:  58     ISSN:  0172-6390     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8007849     Medline TA:  Hepatogastroenterology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1757-1764     Citation Subset:  -    
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