| Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography. | |
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MedLine Citation:
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PMID: 20625737 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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AIMS: To compare in vivo DNA lesions induced during helical and sequential coronary computed tomography angiography (CTA) and to evaluate the effect of CT parameters on double-strand break (DSB) levels. METHODS: Thirty-six patients were examined with various CT protocols and modes (helical scan, n = 27; sequential scan, n = 9) either using a 64-slice dual-source or a 128-slice CT system. Blood samples were obtained before and 30 min after CT. Lymphocytes were isolated, stained against the phosphorylated histone variant γ-H2AX, and DSBs were visualised by using fluorescence microscopy. RESULTS: DSB yields 30 min after CTA ranged from 0.04 to 0.71 per cell and showed a significant correlation to DLP (ρ = 0.81, p < 0.00001). Median DSB yield and median DLP were significantly lower after sequential compared to helical CT examinations (0.11 vs. 0.37 DSBs/cell and 249 vs. 958 mGy cm, p < 0.00001). Additional calcium scoring led to an increase in DLP (p = 0.15) and DSB levels (p = 0.04). DSB levels normalised to the DLP showed a significant correlation to the attenuation of the blood (ρ = 0.53, p = 0.01) and a negative correlation to the body mass index of the patients (ρ = -0.37, p = 0.06). CONCLUSION: γ-H2AX immunofluorescence microscopy allows one to determine dose-related effects on x-ray-induced DSB levels and to consider individual factors which cannot be monitored by physical dose measurements. |
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Authors:
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M A Kuefner; S Grudzenski; J Hamann; S Achenbach; Michael Lell; K Anders; S A Schwab; L Häberle; M Löbrich; M Uder |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-13 |
Journal Detail:
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Title: European radiology Volume: 20 ISSN: 1432-1084 ISO Abbreviation: Eur Radiol Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9114774 Medline TA: Eur Radiol Country: Germany |
Other Details:
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Languages: eng Pagination: 2917-24 Citation Subset: IM |
Affiliation:
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Department of Radiology, University of Erlangen-Nürnberg, Maximiliansplatz 1, 91054, Erlangen, Germany. michael.kuefner@uk-erlangen.de |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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