| Effect of C2 ceramide on the inositol phospholipid metabolism (uptake of 32P, 3H-serine and 3H-palmitic acid) and apoptosis-related morphological changes in Tetrahymena. | |
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MedLine Citation:
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PMID: 10190048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sphingomyelin metabolites have significant role in the regulation of many life processes of mammalian cells. In the present experiments the influence of phospholipid turnover and apoptosis related morphologic signs by one of this metabolite, C2 ceramide was studied, and compared to the control, untreated cells, in the unicellular Tetrahymena. The incorporation of phospholipid head group components (serine, phosphorus) show a clear time-dependence; while the incorporation of fatty acid component (palmitic acid) is very fast: no significant alterations were found between 5- and 60-min incubations. C2 ceramide treatment didn't alter 3H-palmitic acid incorporation into phospholipids, however 3H-serine incorporation was mainly inhibited. The amount of total incorporated 32P was also decreased, on the other hand the lover concentration C2 ceramide (10 microM) elevated the synthesis of inositol phospholipids. The higher concentration of C2 ceramide (50 microM) had inhibitory effect on the synthesis of each phospholipids examined. This means that in the presence of the C2 ceramide the synthesis, recovery and turnover of phospholipids, participating in signal transduction, are altered. However these observations were based the uptake of labeled phospholipid precursors, which gives information on the dynamics of the process, without using lipid mass measurements. C2 ceramide also caused the rounding off the cells, DNA degradation and nuclear condensation. These latter observations point to morphological signs of apoptosis. The results call attention to the role of sphingomyelin metabolites on signalization of unicellulars, to the cross-talk between the inositol phospholipids and sphingomyelin metabolites, and the role of these molecules in the apoptotic processes at a low evolutionary level. |
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Authors:
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P Kovács; H Hegyesi; L Köhidai; P Nemes; G Csaba |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology Volume: 122 ISSN: 1367-8280 ISO Abbreviation: Comp. Biochem. Physiol. C, Pharmacol. Toxicol. Endocrinol. Publication Date: 1999 Feb |
Date Detail:
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Created Date: 1999-05-26 Completed Date: 1999-05-26 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9516060 Medline TA: Comp Biochem Physiol C Pharmacol Toxicol Endocrinol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 215-24 Citation Subset: IM |
Affiliation:
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Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, Budapest, Hungary. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Chromatin / metabolism DNA Fragmentation Kinetics Palmitic Acid / metabolism Phosphatidylinositols / metabolism* Phosphorus Radioisotopes / metabolism Serine / metabolism Sphingosine / analogs & derivatives*, pharmacology Tetrahymena / cytology, drug effects*, metabolism Tritium |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/N-acetylsphingosine; 0/Phosphatidylinositols; 0/Phosphorus Radioisotopes; 10028-17-8/Tritium; 123-78-4/Sphingosine; 56-45-1/Serine; 57-10-3/Palmitic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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