Document Detail


Effect of Bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats.
MedLine Citation:
PMID:  9498277     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of Bifidobacterium longum (4 x 10(8) viable cells/g diet) and a derivative of inulin ('Raftiline HP'; 5% w/w in diet) on colonic aberrant crypt foci (ACF) induced by the colon carcinogen azoxymethane (AOM) has been studied. The concentration of ammonia, a putative tumour promoter produced by bacterial degradation of protein and urea, and the activities of certain bacterial enzymes thought to play a role in colon carcinogenesis, beta-glucuronidase and beta-glucosidase were also assayed. Consumption of either B. longum or inulin was associated with a decrease (26 and 41%, respectively) in AOM-induced small ACF (i.e. those comprising 1-3 aberrant crypts per focus). Combined administration of the bifidobacterium and inulin resulted in more potent inhibition of ACF than administration of the two separately, achieving 80% inhibition of small ACF. Furthermore, the combined administration significantly decreased the incidence (by 59%) of large ACF (>4 aberrant crypts per focus), which are considered to be predictive of eventual tumour incidence. Since the dietary treatments were started 1 week after the carcinogen dose, the results suggest that B. longum and inulin may be affecting the early promotion phase of the carcinogenic process. Consumption of diets containing B. longum, inulin or both were also associated with decreases in beta-glucuronidase activity and ammonia concentration in the caecal contents. Both these factors have been associated with carcinogenesis of the colon in experimental animal models. In rats given inulin-containing diets (with or without B. longum) an increase in caecal wt and beta-glucosidase activity and a decrease in caecal pH were observed. The results suggest that consumption of B. longum or inulin was associated with potentially beneficial changes in caecal physiology and bacterial metabolic activity in relation to tumour risk and in the incidence of putative preneoplastic lesions in the colon. The results also indicated that combined treatment with the two agents was more effective in reducing colonic lesions.
Authors:
I R Rowland; C J Rumney; J T Coutts; L C Lievense
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carcinogenesis     Volume:  19     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-24     Completed Date:  1998-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  281-5     Citation Subset:  IM    
Affiliation:
Northern Ireland Centre for Diet and Health, School of Biomedical Sciences, University of Ulster, Coleraine, UK. I.Rowland@ulst.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Ammonia / metabolism
Animals
Azoxymethane
Bacteria / metabolism*
Bifidobacterium* / metabolism
Cecum / drug effects,  physiology
Colonic Neoplasms / chemically induced,  metabolism,  prevention & control
Glucuronidase / metabolism
Hydrogen-Ion Concentration
Intestinal Mucosa / drug effects,  metabolism*,  microbiology*
Inulin / pharmacology*
Male
Precancerous Conditions / chemically induced,  metabolism*,  prevention & control
Probiotics / pharmacology*
Rats
Rats, Sprague-Dawley
beta-Glucosidase / metabolism
Chemical
Reg. No./Substance:
25843-45-2/Azoxymethane; 7664-41-7/Ammonia; 9005-80-5/Inulin; EC 3.2.1.21/beta-Glucosidase; EC 3.2.1.31/Glucuronidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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