Document Detail

Effect of Bcl-2 and Bax on survival of side population cells from hepatocellular carcinoma cells.
MedLine Citation:
PMID:  18023099     Owner:  NLM     Status:  MEDLINE    
AIM: To understand the role and significance of side population (SP) cells from hepatocellular carcinoma (HCC) in hepatocarcinogenesis, development, relapse and metastasis, we simulated the denutrition conditions that cancer cells experience in clinical therapy, observed the different anti-apoptosis ability of SP cells and non-SP cells under such conditions, and established the possible effects of P53, Bcl-2 and Bax on survival of SP cells. METHODS: We used flow cytometry to analyze and sort the SP and non-SP cells in established HCC lines MHCC97 and hHCC. We evaluated cell proliferation by methyl thiazolyl tetrazolium (MTT) assay and investigated the expression of p53, bcl-2 and bax genes during denutrition, by RT-PCR and immunofluorescence staining. RESULTS: The percentage of SP cells in the two established HCC lines was 0.25% and 0.5%, respectively. SP cells had greater anti-apoptosis and proliferation ability than non-SP cells. Expression of Bcl-2 and Bax in SP and non-SP cells differed during denutrition. The former was up-regulated in SP cells, and the latter was up-regulated in non-SP cells. CONCLUSION: It may be that different upstream molecules acted and led to different expression levels of Bcl-2 and Bax in these two cell lines. There was a direct relationship between up-regulation of Bcl-2 and down-regulation of Bax and higher anti-apoptosis ability in SP cells. It may be that the existence and activity of SP cells are partly responsible for some of the clinical phenomena which are seen in HCC, such as relapse or metastasis. Further research on SP cells may have potential applications in the field of anticancer therapy.
Jing Fan; Ren Li; Rui Zhang; Hai-Liang Liu; Ning Zhang; Fu-Qing Zhang; Ke-Feng Dou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  13     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-20     Completed Date:  2008-03-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  6053-9     Citation Subset:  IM    
Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, 17 Changle Western Road, Xi'an 710032, Shaanxi Province, China.
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MeSH Terms
Carcinoma, Hepatocellular / metabolism,  pathology*
Cell Proliferation
Fluorescent Antibody Technique
Liver Neoplasms / metabolism,  pathology*
Proto-Oncogene Proteins c-bcl-2 / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Tetrazolium Salts / diagnostic use
Thiazoles / diagnostic use
Tumor Suppressor Protein p53 / metabolism*
bcl-2-Associated X Protein / metabolism*
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 0/Tetrazolium Salts; 0/Thiazoles; 0/Tumor Suppressor Protein p53; 0/bcl-2-Associated X Protein; 298-93-1/thiazolyl blue

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