Document Detail

Effect of BCG stimulus on proinflammatory cytokine production in laryngeal cancer.
MedLine Citation:
PMID:  18421458     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Evaluate the production of TNF and IL-6 in the supernatant of peripheral blood mononuclear cell (PBMC) cultures of patients with supraglottic laryngeal cancer before and after surgical treatment. MATERIALS AND METHODS: Adherent cell cultures were stimulated with LPS and BCG. Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cytokine concentration was determined by ELISA in supernatants of mononuclear cell cultures. RESULTS: In non-stimulated cultures, lower TNF cytokine levels were detected during the late postoperative (LP) period compared to control (P = 0.02). LP TNF and IL-6 levels were high in cultures stimulated with LPS compared with the preoperative period (PREOP) (P = 0.007; P = 0.008, respectively). Stimulation with BCG led to increased levels of TNF and IL-6 during the LP period compared to control (P = 0.001; P = 0.04, respectively). CONCLUSION: BCG is able to modulate the immune response of patients with advanced supraglottic laryngeal cancer in vitro, increasing the secretion of TNF and IL-6 by macrophages during the postoperative period.
Luiz Carlos Conti-Freitas; Maria Cristina Foss-Freitas; Rui Celso Martins Mamede; Norma Tiraboschi Foss
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Publication Detail:
Type:  Journal Article     Date:  2008-04-18
Journal Detail:
Title:  Cancer immunology, immunotherapy : CII     Volume:  58     ISSN:  1432-0851     ISO Abbreviation:  Cancer Immunol. Immunother.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-10-27     Completed Date:  2009-01-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8605732     Medline TA:  Cancer Immunol Immunother     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  25-9     Citation Subset:  IM    
Department of Head and Neck Surgery, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil.
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MeSH Terms
BCG Vaccine / immunology,  therapeutic use*
Carcinoma / drug therapy*
Cytokines / biosynthesis*,  blood,  immunology
Interleukin-6 / biosynthesis,  blood,  immunology
Laryngeal Neoplasms / drug therapy*,  immunology
Middle Aged
Neoplasm Staging
Tumor Necrosis Factor-alpha / biosynthesis,  blood,  immunology
Reg. No./Substance:
0/BCG Vaccine; 0/Cytokines; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha

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