Document Detail


Effect of Azadinium spinosum on the feeding behaviour and azaspiracid accumulation of Mytilus edulis.
MedLine Citation:
PMID:  22982497     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Azadinium spinosum, a small toxic dinoflagellate, was recently isolated and identified as a primary producer of azaspiracid toxins (AZAs). Previous experiments related to AZA accumulation in blue mussels upon direct feeding with A. spinosum revealed increased mussel mortality and had negative effects on the thickness of the digestive gland tubules. Therefore we conducted follow up experiments in order to study effects of A. spinosum on mussel feeding behaviour. Individual assessment of mussel feeding time activity (FTA), clearance rate (CR), filtration rate (TFR), absorption rate (AR), faeces and pseudofaeces production were carried out on mussel fed either toxic (A. spinosum) or non-toxic (Isochrisis aff. galbana (T-Iso)) diets. Furthermore, AZA accumulation and biotransformation in mussels were followed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). A. spinosum had a significant effect on mussel feeding behaviour compared to T-Iso: CR was lower by a factor of 6, FTA by a factor of 5, TFR by a factor of 3 and AR even decreased to negative values for the last day of exposure. Even so, a rapid AZA accumulation was observed during the first hours of the trial; less than 6h of feeding were required to reach AZA concentration in mussel above regulatory level. In consistence with physiological observations, AZA concentration of about 200μgkg(-1) did not increase further until the end of the study. AZA bioconversion was also found to be a fast process: after 3h of exposure AZA17, -19 and AZA7-10 were already found, with a proportion of AZA17 equal to AZA2. These results show a negative effect of A. spinosum on blue mussel feeding activity and indicate a possible regulation of AZA uptake by decreasing filtration and increasing pseudofaeces production.
Authors:
Thierry Jauffrais; Andrea Contreras; Christine Herrenknecht; Philippe Truquet; Véronique Séchet; Urban Tillmann; Philipp Hess
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-28
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  124-125C     ISSN:  1879-1514     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-9-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  179-187     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
IFREMER, Laboratoire EMP/PHYC, Rue de l'Ile d'Yeu, 44311 Nantes, France. Electronic address: thierry.jauffrais@ifremer.fr.
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