Document Detail


Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury.
MedLine Citation:
PMID:  20954833     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
NADPH-oxidase is an enzyme responsible for reactive oxygen species production, and inhibition of this enzyme represents an attractive therapeutic target for the treatment of many diseases. The aim of this study was to investigate the effects of Apocynin, NADPH-oxidase inhibitor, in the modulation of secondary injury in the spinal cord. The injury was induced by application of vascular clips to the dura via a four-level T5-T8 laminectomy in mice. Treatment with Apocynin 1 and 6 h after the trauma significantly decreased (1) the degree of spinal cord inflammation and tissue injury, (2) neutrophil infiltration, (3) adhesion molecule expression, (4) nuclear transcription factor-κB expression, (5) nitrotyrosine and poly-ADP-ribose formation, (6) pro-inflammatory cytokines production, (7) apoptosis and (8) mitogen-activated protein kinase activation. Moreover, Apocynin significantly ameliorated the loss of limb function (evaluated by motor recovery score). Thus, it is proposed that Apocynin may be useful in the treatment of inflammation associated with spinal cord trauma.
Authors:
Daniela Impellizzeri; Emanuela Mazzon; Emanuela Esposito; Irene Paterniti; Placido Bramanti; Salvatore Cuzzocrea
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Publication Detail:
Type:  Journal Article     Date:  2010-10-18
Journal Detail:
Title:  Free radical research     Volume:  45     ISSN:  1029-2470     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  221-36     Citation Subset:  IM    
Affiliation:
Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, University of Messina, Italy.
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