Document Detail


Effect of acoustic power on in vivo molecular imaging with targeted microbubbles: implications for low-mechanical index real-time imaging.
MedLine Citation:
PMID:  19910159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The aim of this study was to evaluate the influence of acoustic power on ultrasound molecular imaging data with targeted microbubbles.
METHODS: Imaging was performed with a contrast-specific multipulse method at mechanical indexes (MIs) of 0.18 and 0.97. In vitro imaging was used to measure concentration-intensity relationships and to assess whether damping from microbubble attachment to cultured endothelial cells affected signal enhancement. Power-related differences in signal enhancement were evaluated in vivo by P-selectin-targeted and control microbubble imaging in a murine model of hind-limb ischemia-reperfusion injury.
RESULTS: During in vitro experiments, there was minimal acoustic damping from microbubble-cell attachment at either MI. Signal enhancement in the in vitro and in vivo experiments was 2-fold to 3-fold higher for high-MI imaging compared with low-MI imaging, which was due to greater pixel intensity, the detection of a greater number of retained microbubbles, and increased point-spread function. Yet there was a linear relationship between high-MI and low-MI data indicating that the relative degree of enhancement was similar.
CONCLUSION: During molecular imaging, high-MI protocols produce more robust targeted signal enhancement than low-MI protocols, although differences in relative enhancement caused by condition or agent are similar.
Authors:
Beat A Kaufmann; Chad L Carr; Todd Belcik; Aris Xie; Benjamin Kron; Qi Yue; Jonathan R Lindner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-11-11
Journal Detail:
Title:  Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography     Volume:  23     ISSN:  1097-6795     ISO Abbreviation:  J Am Soc Echocardiogr     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-05-03     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  8801388     Medline TA:  J Am Soc Echocardiogr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  79-85     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Computer Systems
Contrast Media*
Drug Delivery Systems / methods
Endothelial Cells / ultrasonography*
Energy Transfer
Mice
Microbubbles*
Radiation Dosage
Reperfusion Injury / ultrasonography*
Reproducibility of Results
Sensitivity and Specificity
Ultrasonography / methods*
Grant Support
ID/Acronym/Agency:
R01 HL078610/HL/NHLBI NIH HHS; R01 HL078610-05/HL/NHLBI NIH HHS; R01 HL078610-06/HL/NHLBI NIH HHS; R01-DK063508/DK/NIDDK NIH HHS; R01-HL074443/HL/NHLBI NIH HHS; R01-HL078610/HL/NHLBI NIH HHS; RC1 HL100659/HL/NHLBI NIH HHS; RC1 HL100659-01/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Contrast Media
Comments/Corrections

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