| Effect of 7-con-O-methylnogarol on DNA synthesis, survival, and cell cycle progression of Chinese hamster ovary cells. | |
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MedLine Citation:
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PMID: 6458356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of 7-con-O-methylnogarol (7-OMEN) on the survival of exponentially growing and plateau-phase Chinese hamster ovary cells was determined in a cloning assay. After 2 hr of exposure, the 50% lethal dose for exponential and plateau-phase cells was 0.3 and 1.5 microgram/ml, respectively. Drug doses for cell progression studies were based upon drug lethality; therefore, higher doses were used for plateau than for exponential populations. The effect of 7-OMEN on cell progression was studied by DNA flow cytometry under the following conditions: (a) during 24 hr of continuous exposure of exponentially growing cells; (b) during recovery of exponential cells after 2 or 7 hr of drug exposure; and (c) during recovery of plateau-phase cells after 2 hr of exposure. Exponential cells exposed continuously for 24 hr progressed normally from M to G1 phase and from G1 to S phase, progression through S phase was slowed, and cells were ultimately blocked in G2 + M. Inhibition of S-phase progression was dose dependent, 0.2 microgram/ml having only slight effect and 1.0 microgram/ml accumulating a large fraction in S phase. Inhibition of S-phase progression correlated with DNA synthesis inhibition. Similar inhibitory effects were observed after pulsed (2- or 7-hr) exposure of exponential cells. 7-OMEN also blocked plateau-phase cells in G2 + M after 2 hr of exposure, but higher doses (3.0 microgram/ml) were required. Simultaneous exposure of exponential cells to Colcemid (which blocks cells in metaphase) and 1.0 microgram 7-OMEN per ml completely inhibited the expected increase in mitotic index, indicating that the G2 + M block observed by DNA flow cytometry was a block in G2 or prophase. |
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Authors:
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E G Adams; S L Crampton; B K Bhuyan |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cancer research Volume: 41 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1981 Dec |
Date Detail:
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Created Date: 1982-02-25 Completed Date: 1982-02-25 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4981-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents / pharmacology* Cell Cycle / drug effects* Cell Survival / drug effects* Cells, Cultured Cricetinae Cricetulus DNA Replication / drug effects* Daunorubicin / analogs & derivatives* Female Menogaril Nogalamycin / administration & dosage, analogs & derivatives, pharmacology* Ovary Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 1404-15-5/Nogalamycin; 20830-81-3/Daunorubicin; 71628-96-1/Menogaril |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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