Document Detail

Effect of 3,5,3'-triiodo-L-thyronine on amino acid accumulation and membrane potential in Sertoli cells of the rat testis.
MedLine Citation:
PMID:  11488410     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to investigate the effect of T3 on amino acid accumulation and on the membrane potential of Sertoli cells of immature rat testes. Testes of pre-pubertal and pubertal rats were pre-incubated (30 min) in Krebs-Ringer bicarbonate buffer and incubated in the presence of [14C]methylaminoisobutyric acid with and without T3 for 15, 45 and 60 min. The hormone (10(-6) M and 10(-7) M) significantly stimulated amino acid accumulation in 6 and 13-day old rat testes but did not have any effect in neonatal and pubertal animals. T3 produced a dose-dependent hyperpolarizing effect at concentrations of 10(-6) M, 10(-5) M, 2 x 10(-5) M and 10(-4) M. We conclude that T3 induces a membrane hyperpolarization in Sertoli cells and stimulates amino acid accumulation in immature rat testes, demonstrating that the hormone has a rapid plasma membrane action.
F R Silva; L D Leite; K P Barreto; C D'Agostini; A Zamoner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  69     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-08-07     Completed Date:  2001-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  977-86     Citation Subset:  IM    
Departamento de Bioquímica, Centro de Ciências Biológicas, UFSC, Florianópolis, SC, Brasil.
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MeSH Terms
Amino Acids / metabolism*
Calcium / metabolism
Membrane Potentials / drug effects*
Rats, Wistar
Sertoli Cells / drug effects,  metabolism
Sexual Maturation
Testis / drug effects*
Triiodothyronine / pharmacology*
beta-Alanine / analogs & derivatives*,  metabolism
Reg. No./Substance:
0/Amino Acids; 107-95-9/beta-Alanine; 19036-43-2/2,2-dimethyl-beta-alanine; 6893-02-3/Triiodothyronine; 7440-70-2/Calcium

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