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Effect of β-(3,4-Dihydroxyphenyl)Lactic Acid on Oxidative Stress Stimulated by High Glucose Levels in Human Peritoneal Mesothelial Cells.
MedLine Citation:
PMID:  22906267     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the effects of β-(3,4-dihydroxyphenyl)lactic acid on oxidative stress stimulated by high glucose levels in human peritoneal mesothelial cells (HPMCs) in vitro.
METHODS: HPMCs were incubated with 100 mol/l glucose followed by 0.625 - 20 mg/ml β-(3,4-dihydroxyphenyl)lactic acid. Reactive oxygen species (ROS) were quantified by flow cytometry. Relative levels of fibronectin-1 (FN1), collagen-I α(1) (COL1A1), endothelin-1 (EDN1) and haem oxygenase-1 (HMOX1) mRNA and protein were quantified by real-time reverse transcription-polymerase chain reaction and Western blotting, respectively. Absolute levels of FN1 and COLIA1 were quantified by enzyme-linked immunosorbent assay.
RESULTS: β-(3,4Dihydroxyphenyl)lactic acid significantly decreased ROS levels, and EDN1 mRNA and protein levels, in dose- and timedependent manners. HMOX1 mRNA and protein levels were significantly increased by β-(3,4-dihydroxyphenyl)lactic acid in dose-dependent manners. COL1A1 and FN1 mRNA and protein levels were significantly decreased by β-(3,4dihydroxyphenyl)lactic acid in dose- and time-dependent manners.
CONCLUSIONS: β-(3,4-Dihydroxyphenyl)lactic acid inhib ited oxidative stress and reversed increases in FN1 and COLIA1 induced by high glucose levels in HPMCs. This may contribute to a protective role in peritoneal fibrosis.
Authors:
H Zhang; J-W Wang; Y Xu; K Zhang; B Yi; J Sun; Y Liu; X-M Zhang; J-S Liu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of international medical research     Volume:  40     ISSN:  1473-2300     ISO Abbreviation:  J. Int. Med. Res.     Publication Date:  2012  
Date Detail:
Created Date:  2012-08-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0346411     Medline TA:  J Int Med Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  943-53     Citation Subset:  IM    
Affiliation:
Department of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
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