Document Detail

Effect of 30 per cent maternal nutrient restriction from 0.16 to 0.5 gestation on fetal baboon kidney gene expression.
MedLine Citation:
PMID:  16513668     Owner:  NLM     Status:  MEDLINE    
Previous studies in rodents and sheep show that maternal nutrient restriction during pregnancy alters fetal renal development. To date, no studies using fetal baboon RNA with human Affymetrix gene chips have been published. In the present study we have (1) evaluated the specificity of the Affymetrix human gene array 'Laboratory on a Chip' system for use with fetal baboon mRNA and (2) investigated the effects of moderate maternal global nutrient restriction (NR; 70% of ad libitum animals) from early (30 days gestation (dG)) to mid-gestation (90 dG; term = 184 dG) on the fetal baboon kidney. Morphometric and blood measurements were made on 12 non-pregnant baboons before they were bred. All baboons were fed ad libitum until 30 days pregnant, at which time six control baboons continued to feed ad libitum (control - C) while six received 70% of the C diet on a weight adjusted basis. Fetal kidneys were collected following caesarean section at 90 dG, with samples flash frozen and fixed for histological assessment. Fetal hip circumference was decreased in the NR group (68 +/- 2 versus 75 +/- 2 mm), while fetal body weight and all other measurements of fetal size were not different between C and NR at 90 dG. Maternal body weight was decreased in the NR group (12.16 +/- 0.34 versus 13.73 +/- 0.55 kg). Having established the specificity of the Affymetrix system for fetal baboon mRNA, gene expression profiling of fetal kidneys in the context of our maternal nutrient restriction protocol shows that NR resulted in a down-regulation of genes in pathways related to RNA, DNA and protein biosynthesis, metabolism and catabolism. In contrast, genes in cell signal transduction, communication and transport pathways were up-regulated in the NR group. These changes indicate that even a moderate level of maternal global NR impacts fetal renal gene pathways. Our histological assessment of renal structure indicates decreased tubule density within the cortex of NR kidneys compared with controls. The number of glomerular cross-sections per unit area were unaffected by NR, suggesting that tubule tortuosity and/or tubule length was decreased in the NR kidney. Taken together the changes indicate that NR results in accelerated fetal renal differentiation. The negative impact of poor maternal nutrition on the fetal kidney may therefore be in part due to shortening of critical phases of renal growth resulting in decreased functional capacity in later life. These findings may have important implications for postnatal renal function, thereby contributing to the observed increased predisposition to hypertension and renal disease in the offspring of nutrient restricted mothers.
L A Cox; M J Nijland; J S Gilbert; N E Schlabritz-Loutsevitch; G B Hubbard; T J McDonald; R E Shade; P W Nathanielsz
Related Documents :
25132618 - Is metformin ready for prime time in pregnancy? probably not yet.
24147928 - Interventions to prevent adverse fetal programming due to maternal obesity during pregn...
15265238 - Endometrial glands as a source of nutrients, growth factors and cytokines during the fi...
24680198 - Maternal choline supplementation: a nutritional approach for improving offspring health?
24357638 - Toxic effects of maternal zearalenone exposure on uterine capacity and fetal developmen...
1690298 - Antagonists of embryo-derived platelet-activating factor act by inhibiting the ability ...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2006-03-02
Journal Detail:
Title:  The Journal of physiology     Volume:  572     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-04     Completed Date:  2006-05-23     Revised Date:  2013-09-02    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  67-85     Citation Subset:  IM    
Department of Genetics, Southwest Foundation for Biomedical Research, 7620 NW Loop 410, San Antonio, TX 78227-5301, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aging / metabolism*
Animal Nutritional Physiological Phenomena
Fetal Development / physiology
Food Deprivation / physiology
Gene Expression Profiling / methods
Gene Expression Regulation, Developmental / physiology
Gestational Age
Kidney / embryology*,  metabolism*
Maternal Nutritional Physiological Phenomena / physiology*
Papio / embryology*,  metabolism*
Pregnancy, Animal
Proteome / metabolism*
Reproducibility of Results
Sensitivity and Specificity
Tissue Distribution
Grant Support
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effects of divalent cations and spermine on the K+ channel TASK-3 and on the outward current in thal...
Next Document:  Dynamic association between alpha-actinin and beta-integrin regulates contraction of canine tracheal...