Document Detail


Effect of 3-hydroxyanthranilic acid on mushroom tyrosinase activity.
MedLine Citation:
PMID:  9659387     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tyrosinase is a copper containing protein which catalyzes the hydroxylation of monophenols and the oxidation of diphenols to o-quinones. The monophenolase activity of tyrosinase is characterized by a typical lag time. In this paper the influence of 3-hydroxyanthranilic acid on monophenolase activity of tyrosinase is reported. 3-Hydroxyanthranilic acid reduced the lag time of tyrosinase when the enzyme acted on N-acetyl-L-tyrosine and on 4-tert-butylphenol. In the presence of 3-hydroxyanthranilic acid, the reaction product 4-tert-butyl-o-benzoquinone, derived from 4-tert-butylphenol oxidation, was formed at a higher rate than in its absence. The results reported in this paper indicate that 3-hydroxyanthranilic acid could affect the enzymic activity of mushroom tyrosinase probably by acting as a diphenol substrate. A K(m) value of 0.78 mM was calculated for 3-hydroxyanthranilic acid as substrate. When tyrosinase acted on 4-tert-butylphenol, K(m) for 3-hydroxyanthranilic acid as a cofactor was estimated to be 37.5 microM. No effect was observed on the diphenolase activity of the enzyme acting on 4-tert-butylcatechol in the presence of 3-hydroxyanthranilic acid.
Authors:
A Rescigno; E Sanjust; G Soddu; A C Rinaldi; F Sollai; N Curreli; A Rinaldi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1384     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-07-30     Completed Date:  1998-07-30     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  268-76     Citation Subset:  IM    
Affiliation:
Istituto di Chimica Biologica, Università di Cagliari, Italy. rescigno@unica.it
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MeSH Terms
Descriptor/Qualifier:
3-Hydroxyanthranilic Acid / pharmacology*
Basidiomycota / enzymology*
Chromatography, High Pressure Liquid
Free Radical Scavengers / pharmacology*
Fungal Proteins / drug effects,  metabolism*
Monophenol Monooxygenase / drug effects,  isolation & purification,  metabolism*
Tyrosine / analogs & derivatives,  metabolism
Chemical
Reg. No./Substance:
0/Free Radical Scavengers; 0/Fungal Proteins; 537-55-3/N-acetyltyrosine; 548-93-6/3-Hydroxyanthranilic Acid; 55520-40-6/Tyrosine; EC 1.14.18.1/Monophenol Monooxygenase

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