| Effect of 26-oxygenosterols from Ganoderma lucidum and their activity as cholesterol synthesis inhibitors. | |
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MedLine Citation:
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PMID: 16000773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ganoderma lucidum is a medicinal fungus belonging to the Polyporaceae family which has long been known in Japan as Reishi and has been used extensively in traditional Chinese medicine. We report the isolation and identification of the 26-oxygenosterols ganoderol A, ganoderol B, ganoderal A, and ganoderic acid Y and their biological effects on cholesterol synthesis in a human hepatic cell line in vitro. We also investigated the site of inhibition in the cholesterol synthesis pathway. We found that these oxygenated sterols from G. lucidum inhibited cholesterol biosynthesis via conversion of acetate or mevalonate as a precursor of cholesterol. By incorporation of 24,25-dihydro-[24,25-3H2]lanosterol and [3-3H]lathosterol in the presence of ganoderol A, we determined that the point of inhibition of cholesterol synthesis is between lanosterol and lathosterol. These results demonstrate that the lanosterol 14alpha-demethylase, which converts 24,25-dihydrolanosterol to cholesterol, can be inhibited by the 26-oxygenosterols from G. lucidum. These 26-oxygenosterols could lead to novel therapeutic agents that lower blood cholesterol. |
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Authors:
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Hassan Hajjaj; Catherine Mac?; Matthew Roberts; Peter Niederberger; Laurent B Fay |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 71 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-07-07 Completed Date: 2005-09-13 Revised Date: 2010-04-06 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 3653-8 Citation Subset: IM |
Affiliation:
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Nestl? Research Centre, Nestec Ltd., Vers-chez-les-Blanc, P.O. Box 44, 1000 Lausanne 26, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anticholesteremic Agents
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pharmacology* Cell Line Cholesterol / biosynthesis* Cytochrome P-450 Enzyme System / antagonists & inhibitors Gas Chromatography-Mass Spectrometry Humans Lanosterol / analogs & derivatives*, biosynthesis, pharmacology* Liver / cytology, drug effects Magnetic Resonance Spectroscopy Oxidoreductases / antagonists & inhibitors Reishi / growth & development, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Anticholesteremic Agents; 57-88-5/Cholesterol; 79-63-0/Lanosterol; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Oxidoreductases; EC 1.14.13.70/sterol 14-demethylase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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