Document Detail


Effect of 2,3-diphosphoglycerate on O2-dissociation kinetics of hemoglobin and glycosylated hemoglobin using the stopped flow technique and an improved in vitro method for hemoglobin glycosylation.
MedLine Citation:
PMID:  8205371     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies under equilibrium conditions have shown that the oxygen affinity of hemoglobin (Hb) is lowered by 2,3-diphosphoglycerate (2,3-DPG), the physiological allosteric effector in erythrocytes, and enhanced by glycosylation of Hb. The kinetics of oxygen release, as a function of 2,3-DPG and the degree of glycosylation have been determined using the stopped flow method and a new in vitro glycosylation procedure allowing adequate amounts of functionally intact hemoglobin to be obtained. The rate constant k of O2-dissociation in glycosylated Hb (8% HbA1c) was approximately 10% lower than in native Hb (4% HbA1c). The addition of 2,3-DPG in concentrations up to 20 mmol/l resulted in a progressive increase of k from 61.5 +/- 3.3 s-1 to 65.3 ae 4.1 s-1 for native Hb and from 56.8 +/- 5.2 s-1 to 59.4 +/- 4.1 s-1 for glycosylated Hb. We conclude that (a) the degree of glycosylation similar to that found in diabetic patients is responsible for a significant decrease of the oxygen dissociation velocity and (b) 2,3-DPG concentration similar to those occurring in vivo have only a weak effect on the oxygen dissociation velocity.
Authors:
J P Marschner; T Seidlitz; N Rietbrock
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of clinical pharmacology and therapeutics     Volume:  32     ISSN:  0946-1965     ISO Abbreviation:  Int J Clin Pharmacol Ther     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-07-14     Completed Date:  1994-07-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423309     Medline TA:  Int J Clin Pharmacol Ther     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  116-21     Citation Subset:  IM    
Affiliation:
Department of Clinical Pharmacology, University Hospital, Frankfurt/Main.
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MeSH Terms
Descriptor/Qualifier:
2,3-Diphosphoglycerate
Diphosphoglyceric Acids / pharmacology*
Glycosylation
Hemoglobin A, Glycosylated / metabolism*
Humans
Oxygen / metabolism
Oxyhemoglobins / metabolism*
Chemical
Reg. No./Substance:
0/Diphosphoglyceric Acids; 0/Hemoglobin A, Glycosylated; 0/Oxyhemoglobins; 138-81-8/2,3-Diphosphoglycerate; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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