| Effect of 16,16-dimethyl prostaglandin E2 on oxygen uptake and microcirculation in the perfused rat liver. | |
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MedLine Citation:
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PMID: 3093357 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous work demonstrated that collagen deposition in the liver of rats fed a nutritionally deficient diet for 3 to 4 months was diminished markedly by 16,16-dimethyl prostaglandin E2 treatment. In this study, rats were fed a high-fat diet or a high-fat diet deficient in lipotropes for 2 to 4 weeks prior to liver perfusion. Rates of O2 uptake by the liver were not changed by dietary manipulation. Infusion of 16,16-dimethyl prostaglandin E2 (10 microM), however, decreased O2 uptake by the whole organ by 20 to 40% in both groups. O2 tension was measured at the liver surface with a miniature O2 electrode placed alternatively on periportal and pericentral regions of the liver lobule. Mean O2 tensions in both periportal and pericentral regions were reduced 2- to 3-fold during the infusion of 16,16-dimethyl prostaglandin E2 suggesting an action on the microcirculation. This hypothesis was supported by the observation that fluorescein isothiocyanate-dextran fluorescence detected from the liver surface as well as hepatic vascular volume determined by dye dilution techniques were decreased 30 to 50% by 16,16-dimethyl prostaglandin E2. In addition, 16,16-dimethyl prostaglandin E2 increased portal pressure by about 10 mm Hg in a reversible manner. Thus, it is concluded that pharmacological levels of 16,16-dimethyl prostaglandin E2 affects the microcirculation dramatically in the isolated perfused liver. |
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Authors:
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H Meren; F Varin; M Ruwart; R G Thurman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Hepatology (Baltimore, Md.) Volume: 6 ISSN: 0270-9139 ISO Abbreviation: Hepatology Publication Date: 1986 Sep-Oct |
Date Detail:
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Created Date: 1986-11-03 Completed Date: 1986-11-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8302946 Medline TA: Hepatology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 917-21 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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16,16-Dimethylprostaglandin E2
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pharmacology* Animals Diet Fluorescein-5-isothiocyanate Fluoresceins Liver / blood supply, drug effects*, metabolism Microcirculation / drug effects Microelectrodes Oxygen / analysis Oxygen Consumption / drug effects* Perfusion Prostaglandins E, Synthetic / pharmacology* Rats Rats, Inbred Strains Thiocyanates |
| Grant Support | |
ID/Acronym/Agency:
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AA-03624/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Prostaglandins E, Synthetic; 0/Thiocyanates; 3326-32-7/Fluorescein-5-isothiocyanate; 39746-25-3/16,16-Dimethylprostaglandin E2; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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