Document Detail

Effect of 13-hydroperoxyoctadecadienoic acid on 15-hydroxy prostaglandin dehydrogenase activity in rabbit kidney cortex.
MedLine Citation:
PMID:  8210445     Owner:  NLM     Status:  MEDLINE    
The effect of a hydroperoxy adduct of linoleic acid, 13-hydroperoxyoctadecadienoic acid (13-HPODE), on 15-hydroxy prostaglandin dehydrogenase activity in rabbit kidney cortex was examined. 13-HPODE inhibited the 15-hydroxy prostaglandin dehydrogenase activity at concentrations ranging from 1 to 10 microM. The effect was concentration-dependent and the concentration required for 50% inhibition was approximately 3 microM. Linoleic acid and 13-hydroxyoctadecadienoic acid (13-HODE) exhibited weaker inhibition of the enzyme activity than did 13-HPODE (linoleic acid, 30% inhibition at 10 microM; 13-HODE, 45% inhibition at 10 microM). Studies utilizing Fe2+ (a catalyst of peroxide decomposition), and mannitol or dimethylsulfoxide (a hydroxy radical scavenger) revealed that the inhibitory effect of 13-HPODE on the 15-hydroxy prostaglandin dehydrogenase activity is not due to the hydroxy radicals which are expected to be formed from 13-HPODE and that the hydroperoxy functional group is a prerequisite. The inhibition by 13-HPODE was uncompetitive and non-competitive with regard to NAD+ and prostaglandin E2, respectively. These results suggest that 13-HPODE has the potential to modulate the prostaglandin catabolism by affecting the 15-hydroxy prostaglandin dehydrogenase activity.
S Sakuma; Y Fujimoto; H Nakagawa; S Hachiki; H Nishida; T Fujita
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Prostaglandins     Volume:  46     ISSN:  0090-6980     ISO Abbreviation:  Prostaglandins     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1993-10-28     Completed Date:  1993-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0320271     Medline TA:  Prostaglandins     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  157-65     Citation Subset:  IM    
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
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MeSH Terms
Dimethyl Sulfoxide / pharmacology
Ferrous Compounds / pharmacology
Hydroxyprostaglandin Dehydrogenases / antagonists & inhibitors*,  metabolism
Kidney Cortex / drug effects,  enzymology*
Linoleic Acid
Linoleic Acids / pharmacology*
Lipid Peroxides*
Mannitol / pharmacology
Reg. No./Substance:
0/Ferrous Compounds; 0/Linoleic Acids; 0/Lipid Peroxides; 2197-37-7/Linoleic Acid; 23017-93-8/13-hydroperoxy-9,11-octadecadienoic acid; 67-68-5/Dimethyl Sulfoxide; 69-65-8/Mannitol; EC 1.1.1.-/Hydroxyprostaglandin Dehydrogenases; EC dehydrogenase

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