Document Detail

Edaravone protects PC12 cells from ischemic-like injury via attenuating the damage to mitochondria.
MedLine Citation:
PMID:  16909478     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Edaravone had been validated to effectively protect against ischemic injuries. In this study, we investigated the protective effect of edaravone by observing the effects on anti-apoptosis, regulation of Bcl-2/Bax protein expression and recovering from damage to mitochondria after OGD (oxygen-glucose deprivation)-reperfusion. METHODS: Viability of PC12 cells which were injured at different time of OGD injury, was quantified by measuring MTT (2-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide) staining. In addition, PC12 cells' viability was also quantified after their preincubation in different concentration of edaravone for 30 min followed by (OGD). Furthermore, apoptotic population of PC12 cells that reinsulted from OGD-reperfusion with or without preincubation with edaravone was determined by flow cytometer analysis, electron microscope and Hoechst/PI staining. Finally, change of Bcl-2/Bax protein expression was detected by Western blot. RESULTS: (1) The viability of PC12 cells decreased with time (1 - 12 h) after OGD. We regarded the model of OGD 2 h, then replacing DMEM (Dulbecco's Modified Eagle's Medium) for another 24 h as an OGD-reperfusion in this research. Furthermore, most PC12 cells were in the state of apoptosis after OGD-reperfusion. (2) The viability of PC12 cells preincubated with edaravone at high concentrations (1, 0.1, 0.01 micromol/L) increased significantly with edaravone protecting PC12 cells from apoptosis after OGD-reperfusion injury. (3) Furthermore, edaravone attenuates the damage of OGD-reperfusion on mitochondria and regulated Bcl-2/Bax protein imbalance expression after OGD-reperfusion. CONCLUSION: Neuroprotective effects of edaravone on ischemic or other brain injuries may be partly mediated through inhibition of Bcl-2/Bax apoptotic pathways by recovering from the damage of mitochondria.
Ying Song; Meng Li; Ji-cheng Li; Er-qing Wei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Zhejiang University. Science. B     Volume:  7     ISSN:  1673-1581     ISO Abbreviation:  -     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-15     Completed Date:  2006-10-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101236535     Medline TA:  J Zhejiang Univ Sci B     Country:  China    
Other Details:
Languages:  eng     Pagination:  749-56     Citation Subset:  IM    
Department of Cellular Biology, School of Medicine, Zhejiang University, Hangzhou 310031, China.
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MeSH Terms
Antipyrine / analogs & derivatives*,  pharmacology
Apoptosis / drug effects
Flow Cytometry
Ischemia / prevention & control*
Microscopy, Electron
Mitochondria / drug effects*
Neuroprotective Agents / pharmacology*
PC12 Cells
Proto-Oncogene Proteins c-bcl-2 / analysis
bcl-2-Associated X Protein / analysis
Reg. No./Substance:
0/Neuroprotective Agents; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 60-80-0/Antipyrine; 89-25-8/phenylmethylpyrazolone

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