Document Detail


Ectopic expression of mitochondria endonuclease Pnu1p from Schizosaccharomyces pombe induces cell death of the yeast.
MedLine Citation:
PMID:  18047809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endonuclease G (EndoG) is a mitochondrial non-specific nuclease that is highly conserved among the eukaryotes. Although the precise role of EndoG in mitochondria is not yet known, the enzyme is released from the mitochondria and digests nuclear DNA during apoptosis in mammalian cells. Schizosaccharomyces pombe has an EndoG homolog Pnu1p (previously named SpNuc1) that is produced as a precursor protein with a mitochondrial targeting sequence. During the sorting into mitochondria the signal sequence is cleaved to yield the functionally active endonuclease. From the analogy to EndoG, active extramitochondrial Pnu1p may trigger cell killing by degrading nuclear DNA. Here, we tested this possibility by expressing a truncated Pnu1p lacking the signal sequence in the extramitochondrial region of pnu1-deleted cells. The truncated Pnu1p was localized in the cytosol and nuclei of yeast cells. And ectopic expression of active Pnu1p led to cell death with fragmentation of nuclear DNA. This suggests that the Pnu1p is possibly involved in a certain type of yeast cell death via DNA fragmentation. Although expression of human Bak in S. pombe was lethal, Pnu1p nuclease is not necessary for hBak-induced cell death.
Authors:
Kaoru Oda; Nami Kawasaki; Masashi Fukuyama; Shogo Ikeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemistry and molecular biology     Volume:  40     ISSN:  1225-8687     ISO Abbreviation:  J. Biochem. Mol. Biol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-30     Completed Date:  2008-02-12     Revised Date:  2009-07-28    
Medline Journal Info:
Nlm Unique ID:  9702084     Medline TA:  J Biochem Mol Biol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  1095-9     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Faculty of Science, Okayama University of Science, 1-1 Ridaicho, Okayama 700-0005, Japan.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / genetics,  physiology
Base Sequence
DNA Fragmentation
DNA Primers / genetics
DNA, Fungal / genetics
Endonucleases / genetics,  physiology*
Genes, Fungal
Mitochondria / enzymology
Peptide Fragments / genetics,  physiology
Schizosaccharomyces / cytology,  genetics,  physiology*
Schizosaccharomyces pombe Proteins / genetics,  physiology*
bcl-2 Homologous Antagonist-Killer Protein / genetics,  physiology
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Fungal; 0/Peptide Fragments; 0/Schizosaccharomyces pombe Proteins; 0/bcl-2 Homologous Antagonist-Killer Protein; EC 3.1.-/Endonucleases

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