Document Detail

Ectopic expression of maize knotted1 results in the cytokinin-autotrophic growth of cultured tobacco tissues.
MedLine Citation:
PMID:  10872218     Owner:  NLM     Status:  MEDLINE    
The ectopic expression of knotted homologues has cytokinin-like effects on plant morphology. The functional relationship between knotted and cytokinins was investigated in cultures of leaf tissue established from tobacco (Nicotiana tabacum L. cv. Havana 425) plants transformed with the maize knotted1 (kn1) gene regulated by cauliflower mosaic virus 35S RNA expression signals. In contrast to leaf tissues of untransformed plants, leaf tissues of kn1 transformants were capable of sustained, cytokinin-autotrophic growth on auxin-containing medium and resembled the tobacco cytokinin-autotrophic mutants Hl-1 and Hl-2. The concentration of 18 cytokinins was measured in cultures initiated from leaves of three independent kn1 transformants and the Hl-1 and Hl-2 mutants. Although cytokinin contents were variable, the content of several cytokinins in Kn1, Hl-1 and Hl-2 tissue lines was at least 10-fold higher than that of wild-type tobacco tissues and in the range reported for other cytokinin-autotrophic tobacco tissues. These results suggest that the cytokinin-autotrophic growth of Kn1 lines could result from elevated steady-state levels of cytokinins.
A Hewelt; E Prinsen; M Thomas; H Van Onckelen; F Meins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Planta     Volume:  210     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2000 May 
Date Detail:
Created Date:  2001-01-05     Completed Date:  2001-01-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  884-9     Citation Subset:  IM    
Friedrich Miescher Institute, Novartis Research Foundation, Basel, Switzerland.
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MeSH Terms
Adenine / analogs & derivatives,  metabolism,  pharmacology
Caulimovirus / genetics
Culture Techniques
Cytokinins / metabolism*,  pharmacology
Homeodomain Proteins / genetics*,  metabolism
Naphthaleneacetic Acids / metabolism,  pharmacology
Plant Proteins / genetics*,  metabolism
Plants, Toxic*
Promoter Regions, Genetic
RNA, Ribosomal / genetics
Tobacco / genetics,  growth & development,  metabolism*
Zea mays / genetics*
Reg. No./Substance:
0/Cytokinins; 0/Homeodomain Proteins; 0/Kn1 protein, plant; 0/Naphthaleneacetic Acids; 0/Plant Proteins; 0/RNA, Ribosomal; 525-79-1/Kinetin; 73-24-5/Adenine

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