| Ectopic expression of constitutively activated RACB in barley enhances susceptibility to powdery mildew and abiotic stress. | |
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MedLine Citation:
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PMID: 16126850 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Small RAC/ROP-family G proteins regulate development and stress responses in plants. Transient overexpression and RNA interference experiments suggested that the barley (Hordeum vulgare) RAC/ROP protein RACB is involved in susceptibility to the powdery mildew fungus Blumeria graminis f. sp. hordei. We created transgenic barley plants expressing the constitutively activated RACB mutant racb-G15V under control of the maize (Zea mays) ubiquitin 1 promoter. Individuals of the T1 generation expressing racb-G15V were significantly more susceptible to B. graminis when compared to segregating individuals that did not express racb-G15V. Additionally, racb-G15V-expressing plants showed delayed shoot development from the third leaf stage on, downward rolled leaves, and stunted roots. Expression of racb-G15V decreased photosynthetic CO(2)-assimilation rates and transpiration of nonstressed leaves. In contrast, racb-G15V-expressing barley leaves, when detached from water supply, showed increased water loss and enhanced transpiration. Water loss was associated with reduced responsiveness to abscisic acid in regard to transpiration when compared to segregants not expressing racb-G15V. Hence, RACB might be a common signaling element in response to both biotic and abiotic stress. |
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Authors:
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Holger Schultheiss; Götz Hensel; Jafargholi Imani; Sylvia Broeders; Uwe Sonnewald; Karl-Heinz Kogel; Jochen Kumlehn; Ralph Hückelhoven |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-08-26 |
Journal Detail:
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Title: Plant physiology Volume: 139 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-09-16 Completed Date: 2006-01-23 Revised Date: 2010-09-20 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 353-62 Citation Subset: IM |
Affiliation:
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Institute of Phytopathology and Applied Zoology, University of Giessen, Germany. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AJ344223; AY234328; M60175 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Abscisic Acid
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metabolism Ascomycota / physiology* Gene Expression Hordeum / genetics, microbiology, physiology* Molecular Sequence Data Mutation Phenotype Photosynthesis Plant Diseases / microbiology* Plant Proteins / genetics, metabolism* Plants, Genetically Modified Signal Transduction Water / metabolism rho GTP-Binding Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Plant Proteins; 21293-29-8/Abscisic Acid; 7732-18-5/Water; EC 3.6.5.2/rho GTP-Binding Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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