| Ect2, an Ortholog of Drosophila's Pebble, Negatively Regulates Neurite Outgrowth in Neuroblastoma × Glioma Hybrid NG108-15 Cells. | |
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MedLine Citation:
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PMID: 21350944 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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To identify genes required for brain development, we previously performed in vivo RNA interference (RNAi) screening in Drosophila embryos. We identified pebble as a gene that disrupts development of the Drosophila nervous system. Although pebble has been shown to be involved in neuronal development of Drosophila in several screens, the involvement of Ect2, a mammalian ortholog of pebble, in mammalian neuronal development has not been addressed. To examine the role of Ect2 in neuronal differentiation, we performed Ect2 RNAi in the mouse neuroblastoma × rat glioma NG108-15 cell line. Depletion of Ect2 resulted in an increased proportion of binucleate cells and morphological differentiation of NG108-15 cells characterized by the outgrowth of neurites. These morphological changes were correlated with an increased level of acetylcholine esterase mRNA. In addition, expression of Ect2 was decreased in differentiated NG108-15 cells induced by dibutyryl cyclic AMP. These findings indicate that Ect2 negatively regulates the differentiation of NG108-15 cells and suggest that Ect2 may play a role in neuronal differentiation and brain development in vivo. |
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Authors:
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Takahiro Tsuji; Chiharu Higashida; Yasumasa Yoshida; Mohammad Saharul Islam; Mitsuko Dohmoto; Keita Koizumi; Haruhiro Higashida |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-25 |
Journal Detail:
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Title: Cellular and molecular neurobiology Volume: - ISSN: 1573-6830 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8200709 Medline TA: Cell Mol Neurobiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Biophysical Genetics, Graduate School of Medical Science, Kanazawa, 920-8640, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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