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Ecotypic Variability in the Metabolic Response of Seeds to Diurnal Hydration-Dehydration Cycles and Its Relation to Seed Vigor.
MedLine Citation:
PMID:  22156384     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Seeds in the seed-bank experience diurnal cycles of imbibition followed by complete dehydration. These conditions pose a challenge to the regulation of germination. The effect of recurring hydration-dehydration (Hy-Dh) cycles were tested on seeds from four Arabidopsis thaliana accessions [Col-0, Cvi, C24 and Ler]. Diurnal Hy-Dh cycles had a detrimental effect on germination rate and on the final percentage of germination in Col-0, Cvi and C24 ecotypes, but not in Ler ecotype, showing improved vigor following the treatments. Membrane permeability measured by ion conductivity was generally increased following each Hy-Dh cycle and was correlated with changes in the Redox status represented by the GSSG/GSH ratio. Among the ecotypes Col-0 seeds displayed highest membrane permeability, whilst Ler was characterized by the greatest increase in electrical conductivity following Hy-Dh cycles. Following Dh 2 and Dh 3, the respiratory activity of Ler seeds significantly increased in contrast to the other ecotypes, indicative of a dramatic shift in metabolism. These differences were associated with accession-specific content and patterns of change of (i) cell wall related laminaribiose and mannose, (ii) fatty acid composition, specifically of the unsaturated oleic acid and α-linoleic acid, (iii) Asn, ornithine and related polyamine putrescine. Furthermore, in Ler ecotype the content of TCA cycle intermediate fumarate, succinate and malate increased in response to dehydration, as opposed to a decrease in the other three ecotypes. These findings provide a link between seed respiration, energy metabolism, fatty acid β-oxidation, N-mobilization and membrane permeability and the improved germination of Ler seeds following Hy-Dh cycles.
Authors:
Bing Bai; Noga Sikron; Tanya Gendler; Yana Kazachkova; Simon Barak; Gideon Grafi; Inna Khozin-Goldberg; Aaron Fait
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-12
Journal Detail:
Title:  Plant & cell physiology     Volume:  -     ISSN:  1471-9053     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
French Associates Institute for Agriculture & Biotechnology of Drylands, Jacob Blaustein Insts. for Desert Research, Ben-Gurion University of the Negev, 84990 Israel.
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