Document Detail


Ecotype-specific and chromosome-specific expansion of variant centromeric satellites in Arabidopsis thaliana.
MedLine Citation:
PMID:  17033808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite the conserved roles and conserved protein machineries of centromeres, their nucleotide sequences can be highly diverse even among related species. The diversity reflects rapid evolution, but the underlying mechanism is largely unknown. One approach to monitor rapid evolution is examination of intra-specific variation. Here we report variant centromeric satellites of Arabidopsis thaliana found through survey of 103 natural accessions (ecotypes). Among them, a cluster of variant centromeric satellites was detected in one ecotype, Cape Verde Islands (Cvi). Recombinant inbred mapping revealed that the variant satellites are distributed in centromeric region of the chromosome 5 (CEN5) of this ecotype. This apparently recent variant accumulation is associated with large deletion of a pericentromeric region and the expansion of satellite region. The variant satellite was bound to HTR12 (centromeric variant histone H3), although expansion of the satellite was not associated with comparable increase in the HTR12 binding. The results suggest that variant satellites with centromere function can rapidly accumulate in one centromere, supporting the model that the satellite repeats in the array are homogenized by occasional unequal crossing-over, which has a potential to generate an expansion of local sequence variants within a centromere cluster.
Authors:
Hidetaka Ito; Asuka Miura; Kazuya Takashima; Tetsuji Kakutani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-11
Journal Detail:
Title:  Molecular genetics and genomics : MGG     Volume:  277     ISSN:  1617-4615     ISO Abbreviation:  Mol. Genet. Genomics     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-15     Completed Date:  2007-02-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101093320     Medline TA:  Mol Genet Genomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  23-30     Citation Subset:  IM    
Affiliation:
Department of Integrated Genetics, National Institute of Genetics, Mishima, Shizuoka, 411-8540, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / physiology*
Arabidopsis / physiology*
Centromere / genetics*
Chromosome Mapping
Chromosomes, Plant / genetics*
Crossing Over, Genetic
DNA, Plant
DNA, Satellite / genetics*
Evolution, Molecular*
Sequence Deletion
Species Specificity
Chemical
Reg. No./Substance:
0/DNA, Plant; 0/DNA, Satellite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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