Document Detail


Ecology of Vibrio vulnificus in estuarine waters of eastern North Carolina.
MedLine Citation:
PMID:  12788759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
While several studies on the ecology of Vibrio vulnificus in Gulf Coast environments have been reported, there is little information on the distribution of this pathogen in East Coast waters. Thus, we conducted a multiyear study on the ecology of V. vulnificus in estuarine waters of the eastern United States, employing extensive multiple regression analyses to reveal the major environmental factors controlling the presence of this pathogen, and of Vibrio spp., in these environments. Monthly field samplings were conducted between July 2000 and April 2002 at six different estuarine sites along the eastern coast of North Carolina. At each site, water samples were taken and nine physicochemical parameters were measured. V. vulnificus isolates, along with estuarine bacteria, Vibrio spp., Escherichia coli organisms, and total coliforms, were enumerated in samples from each site by using selective media. During the last 6 months of the study, sediment samples were also analyzed for the presence of vibrios, including V. vulnificus. Isolates were confirmed as V. vulnificus by using hemolysin gene PCR or colony hybridization. V. vulnificus was isolated only when water temperatures were between 15 and 27 degrees C, and its presence correlated with water temperature and dissolved oxygen and vibrio levels. Levels of V. vulnificus in sediments were low, and no evidence for an overwintering in this environment was found. Multiple regression analysis indicated that vibrio levels were controlled primarily by temperature, turbidity, and levels of dissolved oxygen, estuarine bacteria, and coliforms. Water temperature accounted for most of the variability in the concentrations of both V. vulnificus (47%) and Vibrio spp. (48%).
Authors:
Courtney S Pfeffer; M Frances Hite; James D Oliver
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  69     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-05     Completed Date:  2003-10-01     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3526-31     Citation Subset:  IM    
Affiliation:
Department of Biology, University of North Carolina at Charlotte, Charlotte, North Carolina 28223, USA.
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MeSH Terms
Descriptor/Qualifier:
Colony Count, Microbial
Culture Media
Ecosystem*
Fresh Water / microbiology*
Hemolysin Proteins / genetics
North Carolina
Polymerase Chain Reaction
Regression Analysis
Seasons
Vibrio vulnificus / classification,  genetics,  growth & development,  isolation & purification*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Hemolysin Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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