| Eclosion hormone stimulates cyclic GMP levels in Manduca sexta nervous tissue via arachidonic acid metabolism with little or no contribution from the production of nitric oxide. | |
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MedLine Citation:
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PMID: 1357096 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The neuropeptide eclosion hormone acts directly on the nervous system of the tobacco hornworm, Manduca sexta, to trigger ecdysis behavior at the end of each molt. Previous studies have shown that the action of eclosion hormone is mediated via the intracellular messenger cyclic GMP. In the present study we have investigated the mechanisms involved in the eclosion hormone-stimulated increases in cyclic GMP. No stimulation of guanylate cyclase was seen in homogenized nervous tissue, suggesting that eclosion hormone does not directly stimulate a membrane-bound form of guanylate cyclase. Nitric oxide synthase inhibitors, N-methylarginine and nitroarginine, had no effect on eclosion hormone-stimulated cyclic GMP levels. By contrast, 4-bromophenacyl bromide, an inhibitor of arachidonic acid release, and nordihydroguaiaretic acid, an inhibitor of arachidonic acid metabolism, almost completely abolished the eclosion hormone-stimulated cyclic GMP increase. We hypothesize that eclosion hormone receptors are coupled to a lipase, activation of which causes the release of arachidonic acid. Either the arachidonic acid directly stimulates the soluble guanylate cyclase or further metabolism of arachidonic acid yields compounds that activate guanylate cyclase. |
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Authors:
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D B Morton; M A Giunta |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neurochemistry Volume: 59 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1992-10-26 Completed Date: 1992-10-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1522-30 Citation Subset: IM |
Affiliation:
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Arizona Research Laboratories, Division of Neurobiology, University of Arizona, Tucson 85721. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / antagonists & inhibitors, metabolism*, pharmacology Citrulline / biosynthesis Cyclic GMP / metabolism* Guanylate Cyclase / metabolism Insect Hormones / pharmacology* Moths / metabolism* Nerve Tissue / metabolism* Nitric Oxide / metabolism* Solubility |
| Grant Support | |
ID/Acronym/Agency:
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NS29740/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Insect Hormones; 10102-43-9/Nitric Oxide; 372-75-8/Citrulline; 506-32-1/Arachidonic Acid; 7665-99-8/Cyclic GMP; 86836-08-0/eclosion hormone; EC 4.6.1.2/Guanylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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