Document Detail


Echinoid regulates tracheal morphology and fusion cell fate in Drosophila.
MedLine Citation:
PMID:  20730906     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Morphogenesis of the Drosophila embryonic trachea involves a stereotyped pattern of epithelial tube branching and fusion. Here, we report unexpected phenotypes resulting from maternal and zygotic (M/Z) loss of the homophilic cell adhesion molecule Echinoid (Ed), as well as the subcellular localization of Ed in the trachea. ed(M/Z) embryos have convoluted trachea reminiscent of septate junction (SJ) and luminal matrix mutants. However, Ed does not localize to SJs, and ed(M/Z) embryos have intact SJs and show normal luminal accumulation of the matrix-modifying protein Vermiform. Surprisingly, tracheal length is not increased in ed(M/Z) mutants, but a previously undescribed combination of reduced intersegmental spacing and deep epidermal grooves produces a convoluted tracheal phenotype. In addition, ed(M/Z) mutants have unique fusion defects involving supernumerary fusion cells, ectopic fusion events and atypical branch breaks. Tracheal-specific expression of Ed rescues these fusion defects, indicating that Ed acts in trachea to control fusion cell fate.
Authors:
Caroline Laplante; Sarah M Paul; Greg J Beitel; Laura A Nilson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  239     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-14     Completed Date:  2011-01-31     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2509-19     Citation Subset:  IM    
Copyright Information:
Developmental Dynamics 239:2509-2519, 2010. © 2010 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Amidohydrolases / genetics,  metabolism
Animals
Cell Adhesion Molecules / genetics,  metabolism*
Cell Fusion*
Drosophila Proteins / genetics,  metabolism*
Drosophila melanogaster* / anatomy & histology,  embryology
Embryo, Nonmammalian / anatomy & histology,  physiology
Morphogenesis / physiology*
Phenotype
Repressor Proteins / genetics,  metabolism*
Trachea / anatomy & histology,  embryology
Wnt1 Protein / metabolism
Grant Support
ID/Acronym/Agency:
5 T32 HL076139-0/HL/NHLBI NIH HHS; IG1-89072//Canadian Institutes of Health Research; R01 GM069540/GM/NIGMS NIH HHS; R01 GM069540/GM/NIGMS NIH HHS; R01 GM069540-05/GM/NIGMS NIH HHS; T32 HL076139-05/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Drosophila Proteins; 0/Repressor Proteins; 0/Wnt1 Protein; 0/dpp protein, Drosophila; 0/echinoid protein, Drosophila; 0/wg protein, Drosophila; EC 3.5.-/Amidohydrolases; EC 3.5.-/vermiform protein, Drosophila
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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