| Echinoid regulates tracheal morphology and fusion cell fate in Drosophila. | |
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MedLine Citation:
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PMID: 20730906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Morphogenesis of the Drosophila embryonic trachea involves a stereotyped pattern of epithelial tube branching and fusion. Here, we report unexpected phenotypes resulting from maternal and zygotic (M/Z) loss of the homophilic cell adhesion molecule Echinoid (Ed), as well as the subcellular localization of Ed in the trachea. ed(M/Z) embryos have convoluted trachea reminiscent of septate junction (SJ) and luminal matrix mutants. However, Ed does not localize to SJs, and ed(M/Z) embryos have intact SJs and show normal luminal accumulation of the matrix-modifying protein Vermiform. Surprisingly, tracheal length is not increased in ed(M/Z) mutants, but a previously undescribed combination of reduced intersegmental spacing and deep epidermal grooves produces a convoluted tracheal phenotype. In addition, ed(M/Z) mutants have unique fusion defects involving supernumerary fusion cells, ectopic fusion events and atypical branch breaks. Tracheal-specific expression of Ed rescues these fusion defects, indicating that Ed acts in trachea to control fusion cell fate. |
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Authors:
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Caroline Laplante; Sarah M Paul; Greg J Beitel; Laura A Nilson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 239 ISSN: 1097-0177 ISO Abbreviation: Dev. Dyn. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-14 Completed Date: 2011-01-31 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 2509-19 Citation Subset: IM |
Copyright Information:
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Developmental Dynamics 239:2509-2519, 2010. © 2010 Wiley-Liss, Inc. |
Affiliation:
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Department of Biology, McGill University, Montréal, QC, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amidohydrolases
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genetics,
metabolism Animals Cell Adhesion Molecules / genetics, metabolism* Cell Fusion* Drosophila Proteins / genetics, metabolism* Drosophila melanogaster* / anatomy & histology, embryology Embryo, Nonmammalian / anatomy & histology, physiology Morphogenesis / physiology* Phenotype Repressor Proteins / genetics, metabolism* Trachea / anatomy & histology, embryology Wnt1 Protein / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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5 T32 HL076139-0/HL/NHLBI NIH HHS; IG1-89072//Canadian Institutes of Health Research; R01 GM069540/GM/NIGMS NIH HHS; R01 GM069540-05/GM/NIGMS NIH HHS; T32 HL076139-05/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Adhesion Molecules; 0/Drosophila Proteins; 0/Repressor Proteins; 0/Wnt1 Protein; 0/dpp protein, Drosophila; 0/echinoid protein, Drosophila; 0/wg protein, Drosophila; EC 3.5.-/Amidohydrolases; EC 3.5.-/vermiform protein, Drosophila |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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