| Echinococcus multilocularis primary cells: improved isolation, small-scale cultivation and RNA interference. | |
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MedLine Citation:
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PMID: 20637246 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study we demonstrate RNA interference mediated knock-down of target gene expression in Echinococcus multilocularis primary cells on both the transcriptional and translational level. In addition, we report on an improved method for generating E. multilocularis primary cell mini-aggregates from in vitro cultivated metacestode vesicles, and on the cultivation of small numbers of small interfering RNA-transfected cells in vitro over an extended period of time. This allows assessments on the effects of RNA interference performed on Echinococcus primary cells with regard to growth, proliferation, differentiation of the parasite and the formation of novel metacestode vesicles in vitro. |
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Authors:
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Markus Spiliotis; Chiaki Mizukami; Yuzaburo Oku; Ferenc Kiss; Klaus Brehm; Bruno Gottstein |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-14 |
Journal Detail:
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Title: Molecular and biochemical parasitology Volume: 174 ISSN: 1872-9428 ISO Abbreviation: Mol. Biochem. Parasitol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-08-23 Completed Date: 2010-11-23 Revised Date: 2011-03-30 |
Medline Journal Info:
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Nlm Unique ID: 8006324 Medline TA: Mol Biochem Parasitol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 83-7 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Institute of Parasitology, University of Berne, Länggass-Strasse 122, CH-3012 Berne, Switzerland. markus.spiliotis@ipa.unibe.ch |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Echinococcus multilocularis / genetics*, growth & development*, isolation & purification Humans Parasitology / methods* RNA Interference* |
| Comments/Corrections | |
Erratum In:
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Mol Biochem Parasitol. 2011 Apr;176(2):138 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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