Document Detail


Echinacoside retards cellular senescence of human fibroblastic cells MRC-5.
MedLine Citation:
PMID:  20099521     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, effects of echinacoside, one of the phenylethanoids isolated from the stems of Cistanches salsa, a Chinese traditional herbal medicine, on human embryo lung fibroblastic MRC-5 cells, was investigated. Activity of cell proliferation was evaluated with Alamar Blue, showing that treatment with echinacoside could retard the senescence. Flow cytometry results show that echinacoside could trigger cells in the G1 phase to enter the S phase and G2 phase, and could improve ROS degradation. The results from comet assay indicate that echinacoside could protect cells from DNA damage, partly elucidating the mechanism of its effects. All of the above results suggest that echinacoside has potential anti-senescence activity.
Authors:
Hong Xie; Hui Zhu; Cong Cheng; Yu Liang; Zhao Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Die Pharmazie     Volume:  64     ISSN:  0031-7144     ISO Abbreviation:  Pharmazie     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2010-01-26     Completed Date:  2010-03-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9800766     Medline TA:  Pharmazie     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  752-4     Citation Subset:  IM    
Affiliation:
Protein Science Key Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, School of Medicine, Tsinghua University, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Cell Aging / drug effects*
Cell Line
Cell Survival
Cistanche
Coloring Agents
Comet Assay
DNA Damage
Fibroblasts / drug effects*,  metabolism
Flow Cytometry
G1 Phase / drug effects
G2 Phase / drug effects
Glycosides / pharmacology*
Humans
Lung / cytology
Reactive Oxygen Species / metabolism
S Phase / drug effects
Chemical
Reg. No./Substance:
0/Coloring Agents; 0/Glycosides; 0/Reactive Oxygen Species; 82854-37-3/echinacoside

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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