Document Detail


Ecdysteroid synthesis and molting by the tobacco hornworm, Manduca sexta, in the absence of prothoracic glands.
MedLine Citation:
PMID:  1932778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When a pair of prothoracic glands (PGs) were removed from Manduca sexta pupae on the day of pupation, the hemolymph ecdysteroid titer remained at a low level. When a portion of the gland pair was extirpated from pupae after the critical period for prothoracicotropic hormone release, the maximum hemolymph ecdysteroid titer was reduced in proportion to the mass of the PGs removed. These findings clearly showed that the PGs in intact pupae are responsible for the elevated ecdysteroid titer required to elicit adult development on schedule. When brains were removed on the day of pupation, the initiation of adult development was delayed for weeks or months. In contrast, pupae whose PGs were removed on the day of pupation initiated development only 7 days late, indicating the existence of an additional source of pupal ecdysteroids. Further, abdomens of male M. sexta that were isolated on the day of pupation initiated adult development spontaneously within 70 days. The implantation of day 0 pupal brains into these isolated abdomens accelerated the initiation of adult development and elicited synchronous adult development. The hemolymph ecdysteroid titer of those isolated abdomens receiving implants of brains increased within 5 days and reached a maximum level of 1.5 micrograms/ml. The analysis of hemolymph ecdysteroids by reverse-phase HPLC revealed that ecdysone was the major moiety and that the ecdysteroid composition was similar to that of normal, intact pupae that had just initiated adult development. These results demonstrate that the PGs are not requisite for adult development. An increased hemolymph ecdysteroid titer was also observed in isolated abdomens from which the testes were removed and in abdomens devoid of their digestive tract. Indeed, in the latter case, the ecdysteroid titer attained much higher levels than those observed for abdomens with intact guts. Despite numerous attempts to identify the tissue(s) in the isolated abdomens responsible for the increase in ecdysteroid titer, its identity remains unknown.
Authors:
S Sakurai; J T Warren; L I Gilbert
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of insect biochemistry and physiology     Volume:  18     ISSN:  0739-4462     ISO Abbreviation:  Arch. Insect Biochem. Physiol.     Publication Date:  1991  
Date Detail:
Created Date:  1991-12-20     Completed Date:  1991-12-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8501752     Medline TA:  Arch Insect Biochem Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13-36     Citation Subset:  IM    
Affiliation:
Department of Biology, University of North Carolina, Chapel Hill.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cholestenones / blood
Chromatography, High Pressure Liquid
Hemolymph / chemistry
Insect Hormones / biosynthesis*,  blood,  physiology
Moths / growth & development*,  metabolism*
Pupa / growth & development,  metabolism
Grant Support
ID/Acronym/Agency:
DK-30118/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Cholestenones; 0/Insect Hormones

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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