Document Detail


Ecdysone signaling is required for proper organization and fluid secretion of stellate cells in the Malpighian tubules of Drosophila melanogaster.
MedLine Citation:
PMID:  20209436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Drosophila development is a tightly regulated process involving metamorphosis of a relatively less mobile larva to a highly motile adult, triggered by secretion of 20-hydroxyecdysone. Under the influence of ecdysone, most of the larval tissues degenerate, while the imaginal cells differentiate and form adult specific structures. Although the larval Malpighian tubules do not seem to be affected by ecdysone during metamorphosis, we show that ecdysone signaling plays an important role in the early development and functioning of Malpighian tubules. Disruption of ecdysone receptor function, using targeted expression of dominant negative ecdysone receptor in stellate cells, results in disruption of organization of Malpighian tubules. The number of stellate cells is reduced in such Malpighian tubules. Further, they get clustered rather than distributed in their characteristic wild type pattern. We also demonstrate that expression of Drosophila integrin protein (DRIP), an aquaporin responsible for trans-cellular water transport, is also reduced in stellate cells when ecdysone signaling is disrupted. Our results show that of the three ecdysone receptor isoforms, only EcR-B2 rescues these phenotypes. A similar pattern of stellate cell clustering and reduced expression of DRIP is observed in ecd(1), a temperature sensitive mutant, under non-permissive conditions. These results suggest that ecdysone signaling is required for proper patterning and functioning of stellate cells and that EcR-B2 may be the primary isoform required for ecdysone signaling in stellate cells.
Authors:
Naveen-Kumar Gautam; Madhu G Tapadia
Related Documents :
2004416 - Ligand-independent activation of the sevenless receptor tyrosine kinase changes the fat...
1618156 - A temporally regulated, diffusible activity is required for rod photoreceptor developme...
17207856 - Btk regulates multiple stages in the development and survival of b-1 cells.
2295086 - Selective silencing of cell communication influences anteroposterior pattern formation ...
24731566 - Role of platelet derived growth factor (pdgf) in reverting neuronal nuclear and soma si...
2257016 - Sensitization of multidrug-resistant mouse ascites hd33 and chinese hamster ovary chrc5...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of developmental biology     Volume:  54     ISSN:  1696-3547     ISO Abbreviation:  Int. J. Dev. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-08     Completed Date:  2010-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8917470     Medline TA:  Int J Dev Biol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  635-42     Citation Subset:  IM    
Affiliation:
Department of Zoology, Banaras Hindu University, Varanasi, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / metabolism
Carrier Proteins / genetics,  metabolism
Drosophila Proteins / genetics,  metabolism*
Drosophila melanogaster / genetics,  growth & development*,  metabolism
Ecdysone / genetics,  metabolism*
Ecdysterone / genetics,  metabolism
Integrins / genetics,  metabolism
Larva / physiology
Malpighian Tubules / cytology,  metabolism*
Metamorphosis, Biological / genetics,  physiology
Phenotype
Protein Isoforms / genetics,  metabolism
Receptors, Steroid / genetics,  metabolism
Signal Transduction / genetics,  physiology*
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Drosophila Proteins; 0/Integrins; 0/Protein Isoforms; 0/Receptors, Steroid; 0/ecdysone receptor; 3604-87-3/Ecdysone; 5289-74-7/Ecdysterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of the gene transformer of Anastrepha on the somatic sexual development of Drosophila.
Next Document:  Both jnk and apoptosis pathways regulate growth and terminalia rotation during Drosophila genital di...