| Ecdysone 20-hydroxylation and 3-epimerization in larvae of the gypsy moth, Lymantria dispar L.: tissue distribution and developmental changes. | |
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MedLine Citation:
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PMID: 12769975 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The potential for ecdysone metabolism was determined for various larval tissues of the gypsy moth, Lymantria dispar. Homogenates of fat body, midguts, and Malpighian tubules, taken on different days during the second half of the fifth instar, were incubated with [(3)H]ecdysone, and the products were analyzed by reversed-phase and normal-phase HPLC. All tissues showed conversion to 20-hydroxyecdysone, and midguts also produced 3-epiecdysone. Ecdysone 20-monooxygenase (E20MO) activity in the fat body increased from a low level on day 5 to a peak on day 11, coinciding with the peak in the hemolymph ecdysteroid titer on the penultimate day of the instar. Midguts and Malpighian tubules showed E20MO activity only during the last 3 or 4days of the instar, with the highest activity also occurring on the penultimate day. For the midguts, the appearance of the E20MO coincided with the transition from larval to pupal tissue. No activity was detected in larval midguts. 3-Epiecdysone formation, however, was mainly found in larval midguts, with only marginal activity detectable in pupal midguts. |
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Authors:
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R A. Bell; G F. Weirich |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Journal of insect physiology Volume: 43 ISSN: 1879-1611 ISO Abbreviation: J. Insect Physiol. Publication Date: 1997 Jul |
Date Detail:
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Created Date: 2003-May-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985080R Medline TA: J Insect Physiol Country: - |
Other Details:
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Languages: ENG Pagination: 643-649 Citation Subset: - |
Affiliation:
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Insect Neurobiology and Hormone Laboratory, ARS, USDA, Beltsville, U.S.A. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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