| Eccentric contractions affect muscle membrane phospholipid fatty acid composition in rats. | |
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MedLine Citation:
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PMID: 11571487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study investigated if prior eccentric contractions, and thus mechanical strain and muscle damage, exert an effect on the muscle membrane phospholipid fatty acid composition in rats, and whether a possible effect could be attenuated by dietary supplements. Twenty-three rats were randomised to three groups who received chow with added fish oil (n = 8), vitamin C (n = 8) or no supplement (n = 7). After 3 weeks of feeding, calf muscles on one side were stimulated electrically during anaesthesia causing eccentric contractions. Two days later the white gastrocnemius, a part of the stimulated calf muscle, was excised from both legs. In the muscles stimulated to contract eccentrically, compared to the control muscles, the proportion of arachidonic acid, C20:4,n-6 (17.7 +/- 0.6; 16.4 +/- 0.4% of total fatty acids, respectively) and docosapentanoeic acid, C22:5,n-3 (2.9 +/- 0.1 and 2.7 +/- 0.1% of total fatty acids, respectively) was uniformly higher across groups (P < 0.02) with no differences between diet groups. The proportion of long chain polyunsaturates was also significantly higher in the eccentrically contracted (39.9 +/- 0.6% of total fatty acids) compared to the control leg (38.2 +/- 0.6% of total fatty acids; P < 0.01). In contrast no differences were observed in the fatty acid composition of the triacylglycerols stored within the muscle. Thus one severe bout of eccentric contractions modulates the fatty acid composition of the muscle membrane phospholipids when compared to a control leg, and supplemental intake of fish oil or vitamin C did not attenuate this effect. |
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Authors:
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J W Helge; K J Therkildsen; T B Jørgensen; B J Wu; L H Storlien; S Asp |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental physiology Volume: 86 ISSN: 0958-0670 ISO Abbreviation: Exp. Physiol. Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2001-09-25 Completed Date: 2002-02-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9002940 Medline TA: Exp Physiol Country: England |
Other Details:
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Languages: eng Pagination: 599-604 Citation Subset: IM |
Affiliation:
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Copenhagen Muscle Research Centre, Department of Human Physiology, University of Copenhagen, Denmark. jhelge@cmrc.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / administration & dosage, pharmacology Ascorbic Acid / administration & dosage, pharmacology Blood Glucose Dietary Fats / administration & dosage Electric Stimulation Fatty Acids / metabolism* Fish Oils / administration & dosage, pharmacology Lactic Acid / blood Male Muscle Contraction / physiology* Muscle, Skeletal / cytology, drug effects, metabolism* Phospholipids / metabolism* Random Allocation Rats Rats, Wistar Stress, Mechanical Triglycerides / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Blood Glucose; 0/Dietary Fats; 0/Fatty Acids; 0/Fish Oils; 0/Phospholipids; 0/Triglycerides; 50-21-5/Lactic Acid; 50-81-7/Ascorbic Acid |
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