Document Detail


Ebp1 isoforms distinctively regulate cell survival and differentiation.
MedLine Citation:
PMID:  16832058     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ebp1, an ErbB3 receptor-binding protein, inhibits the proliferation and induces the differentiation of human cancer cells. Ebp1 binds nuclear Akt and prevents DNA fragmentation by inhibiting caspase-activated DNase. Here, we show that Ebp1 possesses two different isoforms, p48 and p42, which differentially mediate PC12 cell survival and differentiation. The longer-form p48 localizes in both the cytoplasm and the nucleus and suppresses apoptosis, whereas the shorter-form p42 predominantly resides in the cytoplasm and promotes cell differentiation. EGF strongly stimulates p42 to bind ErbB3, and the association depends on PKC-mediated phosphorylation of Ebp1. By contrast, p48 does not bind to ErbB3 regardless of EGF treatment. Overexpression of p48 provokes cell proliferation, which is inhibited by p42. Moreover, nerve growth factor elicits extensive sprouting in p42 stably transfected PC12 cells, whereas p48 cells reveal modest neurite outgrowth. Although mitogen-activated protein kinase cascade remains similar in both cells, Akt is more active in p48 cells than in p42 cells. Thus, Ebp1 might regulate cell survival and differentiation through two distinctive p48 and p42 isoforms.
Authors:
Zhixue Liu; Jee-Yin Ahn; Xia Liu; Keqiang Ye
Related Documents :
7830938 - Nerve growth factor and epidermal growth factor rescue pc12 cells from programmed cell ...
2082008 - Melatonin effects on the cytoskeletal organization of mdck and neuroblastoma n1e-115 ce...
14640978 - Role of transient receptor potential canonical 6 (trpc6) in non-transferrin-bound iron ...
15647748 - Development of an ischemic tolerance model in a pc12 cell line.
22364988 - Long-term durability of a st. jude medical x-cell bioprosthesis.
24233188 - The body printed: how 3-d printing could change the face of modern medicine?and why tha...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-07-10
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  103     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-19     Completed Date:  2006-08-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10917-22     Citation Subset:  IM    
Affiliation:
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 615 Michael Street, Atlanta, GA 30322, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Active Transport, Cell Nucleus
Adaptor Proteins, Signal Transducing / genetics,  metabolism*
Animals
Base Sequence
Cell Differentiation*
Cell Line
Cell Nucleolus
Cell Survival
Conserved Sequence
Humans
Molecular Sequence Data
Molecular Weight
Phosphorylation
Protein Binding
Protein Isoforms / genetics,  metabolism
Protein Kinase C / metabolism
Proto-Oncogene Proteins c-akt / metabolism
Rats
Receptor, erbB-3 / metabolism
Sequence Alignment
Grant Support
ID/Acronym/Agency:
R01 NS045627/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Ebp1 protein, rat; 0/Protein Isoforms; EC 2.7.10.1/Receptor, erbB-3; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.13/Protein Kinase C
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The NF-kappaB regulator Bcl-3 and the BH3-only proteins Bim and Puma control the death of activated ...
Next Document:  Shape-persistent macrocyclic aromatic tetrasulfonamides: Molecules with nanosized cavities and their...